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10.1080/22221751.2019.1701391 http://scihub22266oqcxt.onion/10.1080/22221751.2019.1701391 31859605!6968670!31859605     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\31859605.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Emerg+Microbes+Infect 2020 ; 9 (1): 20-31 Nephropedia Template TP {{tp|p=31859605|t=2020. Genetic manipulation of porcine deltacoronavirus reveals insights into NS6 and NS7 functions: a novel strategy for vaccine design |pdf=|usr=}}{{31859605}} gab.com Text Genetic manipulation of porcine deltacoronavirus reveals insights into NS6 and NS7 functions: a novel strategy for vaccine design JO: Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=31859605 #FOAvip Porcine deltacoronavirus (PDCoV) is an emerging swine coronavirus that causes severe diarrhea, resulting in high mortality in neonatal piglets. Despite widespread outbreaks in many countries, no effective PDCoV vaccines are currently available. Here, we generated, for the first time, a full-length infectious cDNA clone of PDCoV. We further manipulated the infectious clone by replacing the NS6 gene with a green fluorescent protein (GFP) to generate rPDCoV-DeltaNS6-GFP; likewise, rPDCoV-DeltaNS7 was constructed by removing the ATG start codons of the NS7 gene. Growth kinetics studies suggest that rPDCoV-DeltaNS7 could replicate similarly to that of the wild-type PDCoV, whereas rPDCoV-DeltaNS6-GFP exhibited a substantial reduction of viral titer in vitro and in vivo. Piglets inoculated with rPDCoV-DeltaNS7 or wild-type PDCoV showed similar diarrheic scores and pathological injury. In contrast, rPDCoV-DeltaNS6-GFP-infected piglets did not show any clinical signs, indicating that the NS6 protein is an important virulence factor of PDCoV and that the NS6-deficient mutant virus might be a promising live-attenuated vaccine candidate. Taken together, the reverse genetics platform described here not only provides more insights into the role of PDCoV accessory proteins in viral replication and pathogenesis, but also allows the development of novel vaccines against PDCoV infection. Twit Text FOAVip Genetic manipulation of porcine deltacoronavirus reveals insights into NS6 and NS7 functions: a novel strategy for vaccine design ????Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=31859605 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31859605 #FOAvip English Wikipedia <ref>{{Cite pmid|31859605}}</ref> Genetic manipulation of porcine deltacoronavirus reveals insights into NS6 and NS7 functions: a novel strategy for vaccine design #MMPMID31859605 Zhang M; Li W; Zhou P; Liu D; Luo R; Jongkaewwattana A; He Q Emerg Microbes Infect 2020[]; 9 (1): 20-31 PMID31859605show ga Porcine deltacoronavirus (PDCoV) is an emerging swine coronavirus that causes severe diarrhea, resulting in high mortality in neonatal piglets. Despite widespread outbreaks in many countries, no effective PDCoV vaccines are currently available. Here, we generated, for the first time, a full-length infectious cDNA clone of PDCoV. We further manipulated the infectious clone by replacing the NS6 gene with a green fluorescent protein (GFP) to generate rPDCoV-DeltaNS6-GFP; likewise, rPDCoV-DeltaNS7 was constructed by removing the ATG start codons of the NS7 gene. Growth kinetics studies suggest that rPDCoV-DeltaNS7 could replicate similarly to that of the wild-type PDCoV, whereas rPDCoV-DeltaNS6-GFP exhibited a substantial reduction of viral titer in vitro and in vivo. Piglets inoculated with rPDCoV-DeltaNS7 or wild-type PDCoV showed similar diarrheic scores and pathological injury. In contrast, rPDCoV-DeltaNS6-GFP-infected piglets did not show any clinical signs, indicating that the NS6 protein is an important virulence factor of PDCoV and that the NS6-deficient mutant virus might be a promising live-attenuated vaccine candidate. Taken together, the reverse genetics platform described here not only provides more insights into the role of PDCoV accessory proteins in viral replication and pathogenesis, but also allows the development of novel vaccines against PDCoV infection. |Animals[MESH] |Animals, Newborn[MESH] |Communicable Diseases, Emerging/immunology/pathology/veterinary/virology[MESH] |Coronavirus Infections/immunology/pathology/*veterinary/virology[MESH] |Coronavirus/classification/genetics/*physiology[MESH] |Cytokines/immunology[MESH] |DNA, Complementary[MESH] |Genome, Viral[MESH] |Reverse Genetics[MESH] |Sus scrofa[MESH] |Swine[MESH] |Swine Diseases/immunology/pathology/*virology[MESH] |Viral Regulatory and Accessory Proteins/*genetics[MESH] |Viral Vaccines/*genetics[MESH] |Virus Replication[MESH]Genetic manipulation of porcine deltacoronavirus reveals insights into(epmc).pdf DeepDyve Pubget Overpricing