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10.1016/j.micpath.2019.103922 http://scihub22266oqcxt.onion/10.1016/j.micpath.2019.103922 31838173!7126716!31838173     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Microb+Pathog 2020 ; 140 (ä): 103922 Nephropedia Template TP {{tp|p=31838173|t=2020. Isolation and characterization of a variant subgroup GII-a porcine epidemic diarrhea virus strain in China |pdf=|usr=}}{{31838173}} gab.com Text Isolation and characterization of a variant subgroup GII-a porcine epidemic diarrhea virus strain in China JO: Microb Pathog http://www.kidney.de/pget.php?&tw=1&pmid=31838173 #FOAvip BACKGROUND: Highly virulent variants of porcine epidemic diarrhea virus (PEDV) have been closely associated with recent outbreaks of porcine epidemic diarrhea (PED) in China, which have resulted in severe economic losses to the pork industry. METHODS: In the current study, the variant PEDV strain HM2017 was isolated and purified and a viral growth curve was constructed according to the median tissue culture infective dose (TCID50). HM2017 were amplify with RT-PCR and analyzed by phylogeny analysis. Animal pathogenicity experiment was carried to evaluate the HM2017 clinical assessment. RESULTS: Genome-based phylogenetic analysis revealed that PEDV strain HM2017 was clustered into the variant subgroup GII-a that is currently circulating in pig populations in China. The highest median tissue culture infectious dose of strain HM2017 after 15 passages in Vero cells was 1.33 x 10(7) viral particles/mL. Strain HM2017 was highly virulent to suckling piglets, which exhibited clinical symptoms at 12 h post-infection (hpi) (i.e., weight loss at 12-84 hpi, increased body temperatures at 24-48 hpi, high viral loads in the jejunum and ileum, and 100% mortality by 84 hpi). CONCLUSION: The present study reports a variant subgroup GII-a PEDV HM2017 strain in China and characterize its pathogenicity. PEDV strain HM2017 of subgroup GII-a presents a promising vaccine candidate for the control of PED outbreaks in China. Twit Text FOAVip Isolation and characterization of a variant subgroup GII-a porcine epidemic diarrhea virus strain in China ????Microb Pathog http://www.kidney.de/pget.php?&tw=1&pmid=31838173 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31838173 #FOAvip English Wikipedia <ref>{{Cite pmid|31838173}}</ref> Isolation and characterization of a variant subgroup GII-a porcine epidemic diarrhea virus strain in China #MMPMID31838173 Yang D; Su M; Li C; Zhang B; Qi S; Sun D; Yin B Microb Pathog 2020[Mar]; 140 (ä): 103922 PMID31838173show ga BACKGROUND: Highly virulent variants of porcine epidemic diarrhea virus (PEDV) have been closely associated with recent outbreaks of porcine epidemic diarrhea (PED) in China, which have resulted in severe economic losses to the pork industry. METHODS: In the current study, the variant PEDV strain HM2017 was isolated and purified and a viral growth curve was constructed according to the median tissue culture infective dose (TCID50). HM2017 were amplify with RT-PCR and analyzed by phylogeny analysis. Animal pathogenicity experiment was carried to evaluate the HM2017 clinical assessment. RESULTS: Genome-based phylogenetic analysis revealed that PEDV strain HM2017 was clustered into the variant subgroup GII-a that is currently circulating in pig populations in China. The highest median tissue culture infectious dose of strain HM2017 after 15 passages in Vero cells was 1.33 x 10(7) viral particles/mL. Strain HM2017 was highly virulent to suckling piglets, which exhibited clinical symptoms at 12 h post-infection (hpi) (i.e., weight loss at 12-84 hpi, increased body temperatures at 24-48 hpi, high viral loads in the jejunum and ileum, and 100% mortality by 84 hpi). CONCLUSION: The present study reports a variant subgroup GII-a PEDV HM2017 strain in China and characterize its pathogenicity. PEDV strain HM2017 of subgroup GII-a presents a promising vaccine candidate for the control of PED outbreaks in China. |Animals[MESH] |China/epidemiology[MESH] |Chlorocebus aethiops[MESH] |Coronavirus Infections/*veterinary[MESH] |Disease Outbreaks/prevention & control[MESH] |Genome, Viral[MESH] |Phylogeny[MESH] |Porcine epidemic diarrhea virus/genetics/immunology/*isolation & purification/pathogenicity[MESH] |Swine[MESH] |Swine Diseases/virology[MESH] |Vero Cells[MESH]Isolation and characterization of a variant subgroup GII-a porcine epi(epmc).pdf DeepDyve Pubget Overpricing