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10.1186/s13287-019-1493-5 http://scihub22266oqcxt.onion/10.1186/s13287-019-1493-5 31823825!6902488!31823825     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Stem+Cell+Res+Ther 2019 ; 10 (1): 378 Nephropedia Template TP {{tp|p=31823825|t=2019. Magnesium-enriched microenvironment promotes odontogenic differentiation in human dental pulp stem cells by activating ERK/BMP2/Smads signaling |pdf=|usr=}}{{31823825}} gab.com Text Magnesium-enriched microenvironment promotes odontogenic differentiation in human dental pulp stem cells by activating ERK/BMP2/Smads signaling JO: Stem Cell Res Ther http://www.kidney.de/pget.php?&tw=1&pmid=31823825 #FOAvip BACKGROUND: Magnesium (Mg(2+))-enriched microenvironment promotes odontogenic differentiation in human dental pulp stem cells (DPSCs), but the regulatory mechanisms remain undefined. The aim of this work was to assess magnesium's function in the above process and to explore the associated signaling pathway. METHODS: DPSCs underwent culture in odontogenic medium with the addition of 0, 1, 5, or 10 mM MgCl(2). Intracellular Mg(2+) levels in DPSCs were evaluated flow cytometrically using Mag-Fluo-4-AM. Mg(2+)-entry was inhibited by TRPM7 inhibitor 2-aminoethoxydiphenyl borate (2-APB). RNA-Sequencing was carried out for assessing transcriptome alterations in DPSCs during odontogenic differentiation associated with high extracellular Mg(2+). KEGG pathway analysis was performed to determine pathways related to the retrieved differentially expressed genes (DEGs). Immunoblot was performed for assessing magnesium's role and exploring ERK/BMP2/Smads signaling. RESULTS: Mg(2+)-enriched microenvironment promoted odontogenic differentiation in DPSCs via intracellular Mg(2+) increase. Consistently, the positive effect of high extracellular Mg(2+) on odontogenic differentiation in DPSCs was blocked by 2-APB, which reduced Mg(2+) entry. RNA-sequencing identified 734 DEGs related to odontogenic differentiation in DPSCs in the presence of high extracellular Mg(2+). These DEGs participated in many cascades such as MAPK and TGF-beta pathways. Consistently, ERK and BMP2/Smads pathways were activated in DPSCs treated with high extracellular Mg(2+). In agreement, ERK signaling inhibition by U0126 blunted the effect of high extracellular Mg(2+) on mineralization and odontogenic differentiation in DPSCs. Interestingly, BMP2, BMPR1, and phosphorylated Smad1/5/9 were significantly decreased by U0126, indicating that BMP2/Smads acted as downstream of ERK. CONCLUSIONS: Mg(2+)-enriched microenvironment promotes odontogenic differentiation in DPSCs by activating ERK/BMP2/Smads signaling via intracellular Mg(2+) increase. This study revealed that Mg(2+)-enriched microenvironment could be used as a new strategy for dental pulp regeneration. Twit Text FOAVip Magnesium-enriched microenvironment promotes odontogenic differentiation in human dental pulp stem cells by activating ERK/BMP2/Smads signaling ????Stem Cell Res Ther http://www.kidney.de/pget.php?&tw=1&pmid=31823825 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31823825 #FOAvip English Wikipedia <ref>{{Cite pmid|31823825}}</ref> Magnesium-enriched microenvironment promotes odontogenic differentiation in human dental pulp stem cells by activating ERK/BMP2/Smads signaling #MMPMID31823825 Kong Y; Hu X; Zhong Y; Xu K; Wu B; Zheng J Stem Cell Res Ther 2019[Dec]; 10 (1): 378 PMID31823825show ga BACKGROUND: Magnesium (Mg(2+))-enriched microenvironment promotes odontogenic differentiation in human dental pulp stem cells (DPSCs), but the regulatory mechanisms remain undefined. The aim of this work was to assess magnesium's function in the above process and to explore the associated signaling pathway. METHODS: DPSCs underwent culture in odontogenic medium with the addition of 0, 1, 5, or 10 mM MgCl(2). Intracellular Mg(2+) levels in DPSCs were evaluated flow cytometrically using Mag-Fluo-4-AM. Mg(2+)-entry was inhibited by TRPM7 inhibitor 2-aminoethoxydiphenyl borate (2-APB). RNA-Sequencing was carried out for assessing transcriptome alterations in DPSCs during odontogenic differentiation associated with high extracellular Mg(2+). KEGG pathway analysis was performed to determine pathways related to the retrieved differentially expressed genes (DEGs). Immunoblot was performed for assessing magnesium's role and exploring ERK/BMP2/Smads signaling. RESULTS: Mg(2+)-enriched microenvironment promoted odontogenic differentiation in DPSCs via intracellular Mg(2+) increase. Consistently, the positive effect of high extracellular Mg(2+) on odontogenic differentiation in DPSCs was blocked by 2-APB, which reduced Mg(2+) entry. RNA-sequencing identified 734 DEGs related to odontogenic differentiation in DPSCs in the presence of high extracellular Mg(2+). These DEGs participated in many cascades such as MAPK and TGF-beta pathways. Consistently, ERK and BMP2/Smads pathways were activated in DPSCs treated with high extracellular Mg(2+). In agreement, ERK signaling inhibition by U0126 blunted the effect of high extracellular Mg(2+) on mineralization and odontogenic differentiation in DPSCs. Interestingly, BMP2, BMPR1, and phosphorylated Smad1/5/9 were significantly decreased by U0126, indicating that BMP2/Smads acted as downstream of ERK. CONCLUSIONS: Mg(2+)-enriched microenvironment promotes odontogenic differentiation in DPSCs by activating ERK/BMP2/Smads signaling via intracellular Mg(2+) increase. This study revealed that Mg(2+)-enriched microenvironment could be used as a new strategy for dental pulp regeneration. |Bone Morphogenetic Protein 2/metabolism[MESH] |Cell Differentiation/*drug effects[MESH] |Cell Proliferation/drug effects[MESH] |Dental Pulp/cytology[MESH] |Extracellular Signal-Regulated MAP Kinases/metabolism[MESH] |Humans[MESH] |Magnesium/*pharmacology[MESH] |Odontogenesis/*drug effects[MESH] |Signal Transduction/*drug effects[MESH] |Smad Proteins/metabolism[MESH] |Stem Cells/cytology/metabolism[MESH]Magnesium-enriched microenvironment promotes odontogenic differentiati(epmc).pdf DeepDyve Pubget Overpricing