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10.1016/j.virusres.2019.197834 http://scihub22266oqcxt.onion/10.1016/j.virusres.2019.197834 31816342!7114822!31816342     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virus+Res 2020 ; 276 (ä): 197834 Nephropedia Template TP {{tp|p=31816342|t=2020. Identification of the immunodominant neutralizing regions in the spike glycoprotein of porcine deltacoronavirus |pdf=|usr=}}{{31816342}} gab.com Text Identification of the immunodominant neutralizing regions in the spike glycoprotein of porcine deltacoronavirus JO: Virus Res http://www.kidney.de/pget.php?&tw=1&pmid=31816342 #FOAvip Porcine deltacoronavirus (PDCoV), is an emerging enteropathogenic coronavirus in pigs, that poses a novel threat to swine husbandry worldwide. Crucial to halting PDCoV transmission and infection is the development of effective therapies and vaccines. The spike (S) protein of coronavirus is the major target of host neutralizing antibodies, however the immunodominant neutralizing region in the S protein of PDCoV has not been defined. Here, three truncations of the PDCoV S protein were generated, the N-terminal domain of the S1 subunit (NTD, amino acids (aa) 50-286), the C-terminal domain of the S1 subunit (CTD, aa 278-616), and S2 subunit (aa 601-1087). The proteins were expressed using an E. coli expression system. Polyclonal antisera against the three recombinant proteins were produced in rabbits and mice. All three antisera were able to inhibit PDCoV infection in vitro, as determined by virus neutralization assay, fluorescent focus neutralization assay, and plaque-reduction neutralization. The CTD-specific antisera had the most potent PDCoV-neutralizing effect, indicating that the CTD region may contain the major neutralizing epitope(s) in the PDCoV S protein. Based on these findings, CTD may be a promising target for development of an effective vaccine against PDCoV infection in pigs. Twit Text FOAVip Identification of the immunodominant neutralizing regions in the spike glycoprotein of porcine deltacoronavirus ????Virus Res http://www.kidney.de/pget.php?&tw=1&pmid=31816342 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31816342 #FOAvip English Wikipedia <ref>{{Cite pmid|31816342}}</ref> Identification of the immunodominant neutralizing regions in the spike glycoprotein of porcine deltacoronavirus #MMPMID31816342 Chen R; Fu J; Hu J; Li C; Zhao Y; Qu H; Wen X; Cao S; Wen Y; Wu R; Zhao Q; Yan Q; Huang Y; Ma X; Han X; Huang X Virus Res 2020[Jan]; 276 (ä): 197834 PMID31816342show ga Porcine deltacoronavirus (PDCoV), is an emerging enteropathogenic coronavirus in pigs, that poses a novel threat to swine husbandry worldwide. Crucial to halting PDCoV transmission and infection is the development of effective therapies and vaccines. The spike (S) protein of coronavirus is the major target of host neutralizing antibodies, however the immunodominant neutralizing region in the S protein of PDCoV has not been defined. Here, three truncations of the PDCoV S protein were generated, the N-terminal domain of the S1 subunit (NTD, amino acids (aa) 50-286), the C-terminal domain of the S1 subunit (CTD, aa 278-616), and S2 subunit (aa 601-1087). The proteins were expressed using an E. coli expression system. Polyclonal antisera against the three recombinant proteins were produced in rabbits and mice. All three antisera were able to inhibit PDCoV infection in vitro, as determined by virus neutralization assay, fluorescent focus neutralization assay, and plaque-reduction neutralization. The CTD-specific antisera had the most potent PDCoV-neutralizing effect, indicating that the CTD region may contain the major neutralizing epitope(s) in the PDCoV S protein. Based on these findings, CTD may be a promising target for development of an effective vaccine against PDCoV infection in pigs. |Animals[MESH] |Antibodies, Neutralizing/immunology[MESH] |Antibodies, Viral/immunology[MESH] |Antibody Affinity[MESH] |Cell Line[MESH] |Coronavirus Infections/immunology/prevention & control[MESH] |Coronavirus/*immunology[MESH] |Immunodominant Epitopes/*immunology[MESH] |Mice[MESH] |Neutralization Tests[MESH] |Protein Subunits[MESH] |Rabbits[MESH] |Recombinant Proteins/chemistry/genetics/immunology[MESH] |Spike Glycoprotein, Coronavirus/chemistry/genetics/*immunology[MESH]Identification of the immunodominant neutralizing regions in the spike(epmc).pdf DeepDyve Pubget Overpricing