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10.3390/v11111054

http://scihub22266oqcxt.onion/10.3390/v11111054
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31766215!6893544!31766215
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suck abstract from ncbi


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pmid31766215      Viruses 2019 ; 11 (11): ä
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  • Delmarva (DMV/1639) Infectious Bronchitis Virus (IBV) Variants Isolated in Eastern Canada Show Evidence of Recombination #MMPMID31766215
  • Hassan MSH; Ojkic D; Coffin CS; Cork SC; van der Meer F; Abdul-Careem MF
  • Viruses 2019[Nov]; 11 (11): ä PMID31766215show ga
  • Infectious bronchitis virus (IBV) infection in chickens can lead to an economically important disease, namely, infectious bronchitis (IB). New IBV variants are continuously emerging, which complicates vaccination-based IB control. In this study, five IBVs were isolated from clinical samples submitted to a diagnostic laboratory in Ontario, Canada, and subjected to detailed molecular characterization. Analysis of the spike (S)1 gene showed that these five IBVs were highly related to the Delmarva (DMV/1639) strain (~97.0% nucleotide sequence similarity) that was firstly isolated from an IB outbreak in the Delmarva peninsula, United States of America (USA), in 2011. However, the complete genomic sequence analysis showed a 93.5-93.7% similarity with the Connecticut (Conn) vaccine strain, suggesting that Conn-like viruses contributed to the evolution of the five Canadian IBV/DMV isolates. A SimPlot analysis of the complete genomic sequence showed evidence of recombination for at least three different IBV strains, including a Conn vaccine-like strain, a 4/91 vaccine-like strain, and one strain that is yet-unidentified. The unidentified strain may have contributed the genomic regions of the S, 3, and membrane (M) genes of the five Canadian IBV/DMV isolates. The study outcomes add to the existing knowledge about involvement of recombination in IBV evolution.
  • |*Genetic Variation[MESH]
  • |*Recombination, Genetic[MESH]
  • |Animals[MESH]
  • |Base Sequence[MESH]
  • |Canada/epidemiology[MESH]
  • |Chickens[MESH]
  • |Coronavirus Infections/*veterinary[MESH]
  • |Genes, Viral[MESH]
  • |Genome, Viral[MESH]
  • |Genomics/methods[MESH]
  • |Genotype[MESH]
  • |Infectious bronchitis virus/*genetics/*isolation & purification[MESH]
  • |Phylogeny[MESH]
  • |Poultry Diseases/*epidemiology/*virology[MESH]
  • |Public Health Surveillance[MESH]


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