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10.1016/j.jviromet.2019.113772 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2019.113772 31712093!7113866!31712093     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2020 ; 276 (ä): 113772 Nephropedia Template TP {{tp|p=31712093|t=2020. Rapid manipulation of the porcine epidemic diarrhea virus genome by CRISPR/Cas9 technology |pdf=|usr=}}{{31712093}} gab.com Text Rapid manipulation of the porcine epidemic diarrhea virus genome by CRISPR/Cas9 technology JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=31712093 #FOAvip Porcine epidemic diarrhea virus (PEDV) is a highly pathogenic enteric coronavirus causing lethal watery diarrhea in suckling piglets. Reverse genetics is a valuable tool to study the functions of viral genes and to generate vaccine candidates. In this study, a full-length infectious cDNA clone of the highly virulent PEDV strain AJ1102 was assembled in a bacterial artificial chromosome (BAC). The rescued virus (rAJ1102) exhibited similar proliferation characteristics in vitro to the wildtype AJ1102. Using CRISPR/Cas9 technology, a recombinant virus rAJ1102-DeltaORF3-EGFP in which the ORF3 gene was replaced with an EGFP gene, was successfully generated, and its proliferation characteristics were compared with the parental rAJ1102. Importantly, it just took one week to construct the recombinant PEDV rAJ1102-DeltaORF3-EGFP using this method, providing a more efficient platform for PEDV genome manipulation, which could also be applied to other RNA viruses. Twit Text FOAVip Rapid manipulation of the porcine epidemic diarrhea virus genome by CRISPR/Cas9 technology ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=31712093 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31712093 #FOAvip English Wikipedia <ref>{{Cite pmid|31712093}}</ref> Rapid manipulation of the porcine epidemic diarrhea virus genome by CRISPR/Cas9 technology #MMPMID31712093 Peng Q; Fang L; Ding Z; Wang D; Peng G; Xiao S J Virol Methods 2020[Feb]; 276 (ä): 113772 PMID31712093show ga Porcine epidemic diarrhea virus (PEDV) is a highly pathogenic enteric coronavirus causing lethal watery diarrhea in suckling piglets. Reverse genetics is a valuable tool to study the functions of viral genes and to generate vaccine candidates. In this study, a full-length infectious cDNA clone of the highly virulent PEDV strain AJ1102 was assembled in a bacterial artificial chromosome (BAC). The rescued virus (rAJ1102) exhibited similar proliferation characteristics in vitro to the wildtype AJ1102. Using CRISPR/Cas9 technology, a recombinant virus rAJ1102-DeltaORF3-EGFP in which the ORF3 gene was replaced with an EGFP gene, was successfully generated, and its proliferation characteristics were compared with the parental rAJ1102. Importantly, it just took one week to construct the recombinant PEDV rAJ1102-DeltaORF3-EGFP using this method, providing a more efficient platform for PEDV genome manipulation, which could also be applied to other RNA viruses. |*CRISPR-Cas Systems[MESH] |Animals[MESH] |Coronavirus Infections/prevention & control/*virology[MESH] |Genome, Viral[MESH] |Porcine epidemic diarrhea virus/*genetics[MESH] |Recombination, Genetic[MESH] |Swine[MESH] |Swine Diseases/*virology[MESH]Rapid manipulation of the porcine epidemic diarrhea virus genome by CR(epmc).pdf DeepDyve Pubget Overpricing