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10.1002/jcp.29104 http://scihub22266oqcxt.onion/10.1002/jcp.29104 31693182!7166520!31693182     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Cell+Physiol 2020 ; 235 (4): 3280-3291 Nephropedia Template TP {{tp|p=31693182|t=2020. PCBP2 promotes the development of glioma by regulating FHL3/TGF-beta/Smad signaling pathway |pdf=|usr=}}{{31693182}} gab.com Text PCBP2 promotes the development of glioma by regulating FHL3/TGF-beta/Smad signaling pathway JO: J Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=31693182 #FOAvip The purpose of this study was to investigate the role of Poly (C)-binding protein 2 (PCBP2) and the related signaling pathway in glioma progression. Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) were performed to measure PCBP2 messenger RNA and protein expression in glioma tissues or cells. Cell transfection was completed using Lipofectamine 2000. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Transwell assay and flow cytometry assay were used to explore the effects of PCBP2 expression on biological behaviors of glioma cells. Western blot assay was used for the detection of pathway related proteins. Expression of PCBP2 in glioma tissues and cells were higher than that in paracancerous tissues and normal cells (both p < .01). Moreover, the elevated expression of PCBP2 was significantly correlated with tumor size (p = .001) and WHO stage (p = .010). Knockdown of PCBP2 could suppress proliferation, migration and invasion of glioma cells and promote apoptosis. Besides, the expression of transforming growth factor-beta (TGF-beta) pathway related proteins TGF-beta1, p-Smad2 and p-Smad7 were decreased following the downregulation of PCBP2. PCBP2 also inhibited FHL3 expression by binding to FHL3-3'UTR. The inhibition of FHL3 could reverse the antitumor action caused by PCBP2 silencing. In vivo assay, PCBP2 was also found to inhibit the tumor growth of glioma. PCBP2 activates TGF-beta/Smad signaling pathway by inhibiting FHL3 expression, thus promoting the development and progression of glioma. Twit Text FOAVip PCBP2 promotes the development of glioma by regulating FHL3/TGF-beta/Smad signaling pathway ????J Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=31693182 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31693182 #FOAvip English Wikipedia <ref>{{Cite pmid|31693182}}</ref> PCBP2 promotes the development of glioma by regulating FHL3/TGF-beta/Smad signaling pathway #MMPMID31693182 Mao J; Sun Z; Cui Y; Du N; Guo H; Wei J; Hao Z; Zheng L J Cell Physiol 2020[Apr]; 235 (4): 3280-3291 PMID31693182show ga The purpose of this study was to investigate the role of Poly (C)-binding protein 2 (PCBP2) and the related signaling pathway in glioma progression. Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) were performed to measure PCBP2 messenger RNA and protein expression in glioma tissues or cells. Cell transfection was completed using Lipofectamine 2000. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Transwell assay and flow cytometry assay were used to explore the effects of PCBP2 expression on biological behaviors of glioma cells. Western blot assay was used for the detection of pathway related proteins. Expression of PCBP2 in glioma tissues and cells were higher than that in paracancerous tissues and normal cells (both p < .01). Moreover, the elevated expression of PCBP2 was significantly correlated with tumor size (p = .001) and WHO stage (p = .010). Knockdown of PCBP2 could suppress proliferation, migration and invasion of glioma cells and promote apoptosis. Besides, the expression of transforming growth factor-beta (TGF-beta) pathway related proteins TGF-beta1, p-Smad2 and p-Smad7 were decreased following the downregulation of PCBP2. PCBP2 also inhibited FHL3 expression by binding to FHL3-3'UTR. The inhibition of FHL3 could reverse the antitumor action caused by PCBP2 silencing. In vivo assay, PCBP2 was also found to inhibit the tumor growth of glioma. PCBP2 activates TGF-beta/Smad signaling pathway by inhibiting FHL3 expression, thus promoting the development and progression of glioma. |Animals[MESH] |Apoptosis[MESH] |Cell Line, Tumor[MESH] |Cell Proliferation/genetics[MESH] |Female[MESH] |Gene Expression Regulation, Neoplastic/genetics[MESH] |Gene Silencing[MESH] |Glioma/*genetics/pathology[MESH] |Heterografts[MESH] |Humans[MESH] |Intracellular Signaling Peptides and Proteins/*genetics[MESH] |LIM Domain Proteins/*genetics[MESH] |Male[MESH] |Mice[MESH] |Middle Aged[MESH] |RNA-Binding Proteins/*genetics[MESH] |Signal Transduction[MESH] |Smad7 Protein/genetics[MESH]PCBP2 promotes the development of glioma by regulating FHL3/TGF-beta/S(epmc).pdf DeepDyve Pubget Overpricing