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10.1177/1040638719883834 http://scihub22266oqcxt.onion/10.1177/1040638719883834 31650901!6900713!31650901     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Vet+Diagn+Invest 2019 ; 31 (6): 909-912 Nephropedia Template TP {{tp|p=31650901|t=2019. One-step triplex reverse-transcription PCR detection of porcine epidemic diarrhea virus, porcine sapelovirus, and porcine sapovirus |pdf=|usr=}}{{31650901}} gab.com Text One-step triplex reverse-transcription PCR detection of porcine epidemic diarrhea virus, porcine sapelovirus, and porcine sapovirus JO: J Vet Diagn Invest http://www.kidney.de/pget.php?&tw=1&pmid=31650901 #FOAvip Swine diarrhea can be caused by multiple agents, including porcine epidemic diarrhea virus (PEDV), porcine sapelovirus (PSV), and porcine sapovirus (SaV). We designed a one-step triplex reverse-transcription PCR (RT-PCR) detection method including 3 pairs of primers that focused on the S1 gene of PEDV, a conserved gene of PSV, and the VP1 gene of SaV. The optimal concentrations of upstream and downstream primers in the triplex RT-PCR were 0.24 muM for PEDV, 0.15 muM for PSV, and 0.2 muM for SaV, and the optimal annealing temperature was 55.5 degrees C. Triplex RT-PCR assessment of 402 piglet diarrhea samples was compared with conventional individual RT-PCR. Concordance rates in both tests for individual viruses were 100%, 97.6%, and 94.4% for PEDV, PSV, and SaV, respectively. PEDV, PSV, and SaV were detected in 57.2%, 10.4%, and 9.0% of the samples, respectively. The high sensitivity and specificity of this triplex RT-PCR-based detection method for PEDV, PSV, and SaV could allow rapid detection and analysis of mixed infections by these 3 viruses. Twit Text FOAVip One-step triplex reverse-transcription PCR detection of porcine epidemic diarrhea virus, porcine sapelovirus, and porcine sapovirus ????J Vet Diagn Invest http://www.kidney.de/pget.php?&tw=1&pmid=31650901 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31650901 #FOAvip English Wikipedia <ref>{{Cite pmid|31650901}}</ref> One-step triplex reverse-transcription PCR detection of porcine epidemic diarrhea virus, porcine sapelovirus, and porcine sapovirus #MMPMID31650901 Jiang C; He H; Zhang C; Zhang X; Han J; Zhang H; Luo Y; Wu Y; Wang Y; Ge B; Xu J J Vet Diagn Invest 2019[Nov]; 31 (6): 909-912 PMID31650901show ga Swine diarrhea can be caused by multiple agents, including porcine epidemic diarrhea virus (PEDV), porcine sapelovirus (PSV), and porcine sapovirus (SaV). We designed a one-step triplex reverse-transcription PCR (RT-PCR) detection method including 3 pairs of primers that focused on the S1 gene of PEDV, a conserved gene of PSV, and the VP1 gene of SaV. The optimal concentrations of upstream and downstream primers in the triplex RT-PCR were 0.24 muM for PEDV, 0.15 muM for PSV, and 0.2 muM for SaV, and the optimal annealing temperature was 55.5 degrees C. Triplex RT-PCR assessment of 402 piglet diarrhea samples was compared with conventional individual RT-PCR. Concordance rates in both tests for individual viruses were 100%, 97.6%, and 94.4% for PEDV, PSV, and SaV, respectively. PEDV, PSV, and SaV were detected in 57.2%, 10.4%, and 9.0% of the samples, respectively. The high sensitivity and specificity of this triplex RT-PCR-based detection method for PEDV, PSV, and SaV could allow rapid detection and analysis of mixed infections by these 3 viruses. |Animals[MESH] |Caliciviridae Infections/diagnosis/*veterinary[MESH] |Coronavirus Infections/diagnosis/*veterinary[MESH] |Diarrhea/diagnosis/*veterinary[MESH] |Multiplex Polymerase Chain Reaction/methods/*veterinary[MESH] |Picornaviridae Infections/diagnosis/*veterinary[MESH] |Picornaviridae/isolation & purification[MESH] |Porcine epidemic diarrhea virus/isolation & purification[MESH] |Reverse Transcriptase Polymerase Chain Reaction/methods/*veterinary[MESH] |Sapovirus/isolation & purification[MESH] |Swine[MESH]One-step triplex reverse-transcription PCR detection of porcine epidem(epmc).pdf DeepDyve Pubget Overpricing