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10.1093/ndt/gfz190 http://scihub22266oqcxt.onion/10.1093/ndt/gfz190 31605492!7203562!31605492     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nephrol+Dial+Transplant 2020 ; 35 (5): 765-773 Nephropedia Template TP {{tp|p=31605492|t=2020. Calciprotein particle inhibition explains magnesium-mediated protection against vascular calcification |pdf=|usr=}}{{31605492}} gab.com Text Calciprotein particle inhibition explains magnesium-mediated protection against vascular calcification JO: Nephrol Dial Transplant http://www.kidney.de/pget.php?&tw=1&pmid=31605492 #FOAvip BACKGROUND: Phosphate (Pi) toxicity is a strong determinant of vascular calcification development in chronic kidney disease (CKD). Magnesium (Mg2+) may improve cardiovascular risk via vascular calcification. The mechanism by which Mg2+ counteracts vascular calcification remains incompletely described. Here we investigated the effects of Mg2+ on Pi and secondary crystalline calciprotein particles (CPP2)-induced calcification and crystal maturation. METHODS: Vascular smooth muscle cells (VSMCs) were treated with high Pi or CPP2 and supplemented with Mg2+ to study cellular calcification. The effect of Mg2+ on CPP maturation, morphology and composition was studied by medium absorbance, electron microscopy and energy dispersive spectroscopy. To translate our findings to CKD patients, the effects of Mg2+ on calcification propensity (T50) were measured in sera from CKD patients and healthy controls. RESULTS: Mg2+ supplementation prevented Pi-induced calcification in VSMCs. Mg2+ dose-dependently delayed the maturation of primary CPP1 to CPP2 in vitro. Mg2+ did not prevent calcification and associated gene and protein expression when added to already formed CPP2. Confirmatory experiments in human serum demonstrated that the addition of 0.2 mmol/L Mg2+ increased T50 from healthy controls by 51 +/- 15 min (P < 0.05) and CKD patients by 44 +/- 13 min (P < 0.05). Each further 0.2 mmol/L addition of Mg2+ led to further increases in both groups. CONCLUSIONS: Our results demonstrate that crystalline CPP2 mediates Pi-induced calcification in VSMCs. In vitro, Mg2+ delays crystalline CPP2 formation and thereby prevents Pi-induced calcification. Twit Text FOAVip Calciprotein particle inhibition explains magnesium-mediated protection against vascular calcification ????Nephrol Dial Transplant http://www.kidney.de/pget.php?&tw=1&pmid=31605492 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31605492 #FOAvip English Wikipedia <ref>{{Cite pmid|31605492}}</ref> Calciprotein particle inhibition explains magnesium-mediated protection against vascular calcification #MMPMID31605492 Ter Braake AD; Eelderink C; Zeper LW; Pasch A; Bakker SJL; de Borst MH; Hoenderop JGJ; de Baaij JHF Nephrol Dial Transplant 2020[May]; 35 (5): 765-773 PMID31605492show ga BACKGROUND: Phosphate (Pi) toxicity is a strong determinant of vascular calcification development in chronic kidney disease (CKD). Magnesium (Mg2+) may improve cardiovascular risk via vascular calcification. The mechanism by which Mg2+ counteracts vascular calcification remains incompletely described. Here we investigated the effects of Mg2+ on Pi and secondary crystalline calciprotein particles (CPP2)-induced calcification and crystal maturation. METHODS: Vascular smooth muscle cells (VSMCs) were treated with high Pi or CPP2 and supplemented with Mg2+ to study cellular calcification. The effect of Mg2+ on CPP maturation, morphology and composition was studied by medium absorbance, electron microscopy and energy dispersive spectroscopy. To translate our findings to CKD patients, the effects of Mg2+ on calcification propensity (T50) were measured in sera from CKD patients and healthy controls. RESULTS: Mg2+ supplementation prevented Pi-induced calcification in VSMCs. Mg2+ dose-dependently delayed the maturation of primary CPP1 to CPP2 in vitro. Mg2+ did not prevent calcification and associated gene and protein expression when added to already formed CPP2. Confirmatory experiments in human serum demonstrated that the addition of 0.2 mmol/L Mg2+ increased T50 from healthy controls by 51 +/- 15 min (P < 0.05) and CKD patients by 44 +/- 13 min (P < 0.05). Each further 0.2 mmol/L addition of Mg2+ led to further increases in both groups. CONCLUSIONS: Our results demonstrate that crystalline CPP2 mediates Pi-induced calcification in VSMCs. In vitro, Mg2+ delays crystalline CPP2 formation and thereby prevents Pi-induced calcification. |*Dietary Supplements[MESH] |Calcium Phosphates/*metabolism[MESH] |Cells, Cultured[MESH] |Humans[MESH] |Magnesium/*pharmacology[MESH] |Myocytes, Smooth Muscle/*drug effects/metabolism[MESH] |Vascular Calcification/metabolism/*prevention & control[MESH]Calciprotein particle inhibition explains magnesium-mediated protectio(epmc).pdf DeepDyve Pubget Overpricing