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10.1016/j.actatropica.2019.105209

http://scihub22266oqcxt.onion/10.1016/j.actatropica.2019.105209
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31600524!ä!31600524

suck abstract from ncbi


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pmid31600524      Acta+Trop 2020 ; 201 (ä): 105209
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  • Vancomycin and florfenicol resistant Enterococcus faecalis and Enterococcus faecium isolated from human urine in an Egyptian urban-rural community #MMPMID31600524
  • Osman K; Zolnikov TR; Badr J; Naim H; Hanafy M; Saad A; Elbehiry A
  • Acta Trop 2020[Jan]; 201 (ä): 105209 PMID31600524show ga
  • Multidrug resistance is one of the top three threats to global public health. Understanding resistance of bacteria is important to help decrease resistance and improve the development of novel antimicrobial agents or other alternative tools to combat public health challenges. Thus, the goal of this study was to investigate the vancomycin and florfenicol resistance genes of five E. faecalis and 15 E. faecium isolated from patients with urinary tract infections. There were 20 Enterococcus obtained from the library collection of randomly selected private hospitals located in the city of El Qanater El Khayreya; these samples were isolated during 2017. Samples were evaluated for their phenotypic characterization of virulence factors, antimicrobial resistance and PCR was conducted to detect the prescence of the vancomycin vanABC and florfenicol resistance genes encoding the catAB, fexAB and cfu. There were six different antibiotic resistance profiles observed. The 20 isolates showed resistance to clindamycin, oxytetracycline and gentamycin. Resistance was evident to ciprofloxacin, norfloxacin and florfenicol in the absence of the cfr gene in all of the 20 Enterococcus isolates. In addition, all isolates produced biofilms and were classified as extensive drug resistant. MAR(indices) of the isolates were >0.6. The MAR(index) of human isolates of enterococci suggest these pathogens originate from a high-risk source of contamination where antibiotics are often used. This information highlights a possible public health concern to the Egyptian community. The results also suggest the emergence of a linezolid sensitive-vancomycin resistant E. faecium and E. faecalis in the absence of the cfr gene.
  • |Anti-Bacterial Agents/*therapeutic use[MESH]
  • |Drug Resistance, Microbial/*drug effects/*genetics[MESH]
  • |Egypt[MESH]
  • |Enterococcus faecalis/*drug effects[MESH]
  • |Enterococcus faecium/*drug effects[MESH]
  • |Humans[MESH]
  • |Microbial Sensitivity Tests[MESH]
  • |Rural Population/statistics & numerical data[MESH]
  • |Thiamphenicol/*analogs & derivatives/therapeutic use[MESH]
  • |Urban Population/statistics & numerical data[MESH]
  • |Urinary Tract Infections/*microbiology[MESH]
  • |Vancomycin/*therapeutic use[MESH]


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