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10.1016/j.virol.2019.09.009 http://scihub22266oqcxt.onion/10.1016/j.virol.2019.09.009 31580972!7112038!31580972     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virology 2019 ; 538 (ä): 61-70 Nephropedia Template TP {{tp|p=31580972|t=2019. Attenuation of a virulent swine acute diarrhea syndrome coronavirus strain via cell culture passage |pdf=|usr=}}{{31580972}} gab.com Text Attenuation of a virulent swine acute diarrhea syndrome coronavirus strain via cell culture passage JO: Virology http://www.kidney.de/pget.php?&tw=1&pmid=31580972 #FOAvip Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a newly identified enteric alphacoronavirus that causes fatal diarrhea in newborn piglets in China. Here, we propagated a virulent strain SADS-CoV/CN/GDWT/2017 in Vero cells for up to 83 passages. Four strains of SADS-CoV/GDWT-P7, -P18, -P48 and -P83 were isolated and characterized. Sequence alignments showed that these four novel strains exhibited 16 nucleotide mutations and resultant 10 amino acid substitutions in open reading frame 1a/1b, spike, NS3a, envelope, membrane and nucleocapsid proteins. Furthermore, a 58-bp deletion in NS7a/7b was found in P48 and P83 strains, which led to the loss of NS7b and 38 amino acid changes of NS7a. Pig infection studies showed that the P7 strain caused typical watery diarrhea, while the P83 strain induced none-to-mild, delayed and transient diarrhea. This is the first report on cell adaption of a virulent SADS-CoV strain, which gives information on the potential virulence determinants of SADS-CoV. Twit Text FOAVip Attenuation of a virulent swine acute diarrhea syndrome coronavirus strain via cell culture passage ????Virology http://www.kidney.de/pget.php?&tw=1&pmid=31580972 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31580972 #FOAvip English Wikipedia <ref>{{Cite pmid|31580972}}</ref> Attenuation of a virulent swine acute diarrhea syndrome coronavirus strain via cell culture passage #MMPMID31580972 Sun Y; Cheng J; Luo Y; Yan XL; Wu ZX; He LL; Tan YR; Zhou ZH; Li QN; Zhou L; Wu RT; Lan T; Ma JY Virology 2019[Dec]; 538 (ä): 61-70 PMID31580972show ga Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a newly identified enteric alphacoronavirus that causes fatal diarrhea in newborn piglets in China. Here, we propagated a virulent strain SADS-CoV/CN/GDWT/2017 in Vero cells for up to 83 passages. Four strains of SADS-CoV/GDWT-P7, -P18, -P48 and -P83 were isolated and characterized. Sequence alignments showed that these four novel strains exhibited 16 nucleotide mutations and resultant 10 amino acid substitutions in open reading frame 1a/1b, spike, NS3a, envelope, membrane and nucleocapsid proteins. Furthermore, a 58-bp deletion in NS7a/7b was found in P48 and P83 strains, which led to the loss of NS7b and 38 amino acid changes of NS7a. Pig infection studies showed that the P7 strain caused typical watery diarrhea, while the P83 strain induced none-to-mild, delayed and transient diarrhea. This is the first report on cell adaption of a virulent SADS-CoV strain, which gives information on the potential virulence determinants of SADS-CoV. |Alphacoronavirus/genetics/growth & development/metabolism/*pathogenicity[MESH] |Animals[MESH] |Chlorocebus aethiops[MESH] |Coronavirus Infections/*veterinary/virology[MESH] |Diarrhea/veterinary/virology[MESH] |Phylogeny[MESH] |Serial Passage[MESH] |Swine[MESH] |Swine Diseases/*virology[MESH] |Vero Cells[MESH] |Viral Proteins/genetics[MESH] |Virulence[MESH]Attenuation of a virulent swine acute diarrhea syndrome coronavirus st(epmc).pdf DeepDyve Pubget Overpricing