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10.1016/j.virusres.2019.197767

http://scihub22266oqcxt.onion/10.1016/j.virusres.2019.197767
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31560964!7114872!31560964
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suck abstract from ncbi


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pmid31560964      Virus+Res 2019 ; 273 (ä): 197767
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  • Antiviral activity of Sambucus FormosanaNakai ethanol extract and related phenolic acid constituents against human coronavirus NL63 #MMPMID31560964
  • Weng JR; Lin CS; Lai HC; Lin YP; Wang CY; Tsai YC; Wu KC; Huang SH; Lin CW
  • Virus Res 2019[Nov]; 273 (ä): 197767 PMID31560964show ga
  • Human coronavirus NL63 (HCoV-NL63), one of the main circulating HCoVs worldwide, causes respiratory tract illnesses like runny nose, cough, bronchiolitis and pneumonia. Recently, a severe respiratory illness outbreak of HCoV-NL63?has been reported in a long-term care facility. Sambucus FormosanaNakai, a species of elderberry, is a traditional medicinal herb with anti-inflammatory and antiviral potential. The study investigated the antiviral activity of Sambucus FormosanaNakai stem ethanol extract and some phenolic acid constituents against HCoV-NL63. The extract was less cytotoxic and concentration-dependently increased anti-HCoV-NL63 activities, including cytopathicity, sub-G1 fraction, virus yield (IC50?=?1.17?mug/ml), plaque formation (IC50?=?4.67?mug/ml) and virus attachment (IC50?=?15.75?mug/ml). Among the phenolic acid constituents in Sambucus FormosanaNakai extract, caffeic acid, chlorogenic acid and gallic acid sustained the anti-HCoV-NL63 activity that was ranked in the following order of virus yield reduction: caffeic acid (IC(50)?=?3.54?muM)?>?chlorogenic acid (IC(50)?=?43.45?muM)?>?coumaric acid (IC(50)?=?71.48?muM). Caffeic acid significantly inhibited the replication of HCoV-NL63 in a cell-type independent manner, and specifically blocked virus attachment (IC(50)?=?8.1?muM). Therefore, the results revealed that Sambucus Formosana Nakai stem ethanol extract displayed the strong anti-HCoV-NL63 potential; caffeic acid could be the vital component with anti-HCoV-NL63 activity. The finding could be helpful for developing antivirals against HCoV-NL63.
  • |Animals[MESH]
  • |Antiviral Agents/*pharmacology[MESH]
  • |Cell Line[MESH]
  • |Cell Survival/drug effects[MESH]
  • |Coronavirus Infections[MESH]
  • |Coronavirus NL63, Human/*drug effects[MESH]
  • |Epithelial Cells/*drug effects/virology[MESH]
  • |Humans[MESH]
  • |Hydroxybenzoates/chemistry/*pharmacology[MESH]
  • |Inhibitory Concentration 50[MESH]
  • |Kidney/cytology/virology[MESH]
  • |Macaca mulatta[MESH]
  • |Plant Extracts/chemistry/*pharmacology[MESH]
  • |Plant Stems/chemistry[MESH]
  • |Respiratory System/cytology/virology[MESH]
  • |Sambucus/*chemistry[MESH]


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