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10.1016/j.virusres.2019.197752 http://scihub22266oqcxt.onion/10.1016/j.virusres.2019.197752 31518629!7114574!31518629     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virus+Res 2019 ; 273 (ä): 197752 Nephropedia Template TP {{tp|p=31518629|t=2019. Epitope mapping and cellular localization of swine acute diarrhea syndrome coronavirus nucleocapsid protein using a novel monoclonal antibody |pdf=|usr=}}{{31518629}} gab.com Text Epitope mapping and cellular localization of swine acute diarrhea syndrome coronavirus nucleocapsid protein using a novel monoclonal antibody JO: Virus Res http://www.kidney.de/pget.php?&tw=1&pmid=31518629 #FOAvip A swine acute diarrhea syndrome coronavirus (SADS-CoV) that causes severe diarrhea in suckling piglets was identified in Southern China in 2017. To develop an antigen that is specific, sensitive, and easy to prepare for serological diagnosis, antigenic sites in the SADS-CoV nucleocapsid (N) protein were screened. We generated and characterized an N-reactive monoclonal antibody (mAb) 3E9 from mice immunized with recombinant N protein. Through fine epitope mapping of mAb 3E9 using a panel of eukaryotic-expressed polypeptides with GFP-tags, we identified the motif (343)DAPVFTPAP(351) as the minimal unit of the linear B-cell epitope recognized by mAb 3E9. Protein sequence alignment indicated that (343)DAPVFTPAP(351) was highly conserved in different SADS-CoV strains and SADS-related coronaviruses from bat, with one substitution in this motif in HKU2-related bat coronavirus. Using mAb 3E9, we observed that N protein was expressed in the cytoplasm and was in the nucleolus during SADS-CoV replication. N protein was immunoprecipitated from SADS-CoV-infected Vero E6 cells. Taken together, our results indicated that 3E9 mAb could be a useful tool to investigate the structure and function of N protein during viral replication. Twit Text FOAVip Epitope mapping and cellular localization of swine acute diarrhea syndrome coronavirus nucleocapsid protein using a novel monoclonal antibody ????Virus Res http://www.kidney.de/pget.php?&tw=1&pmid=31518629 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31518629 #FOAvip English Wikipedia <ref>{{Cite pmid|31518629}}</ref> Epitope mapping and cellular localization of swine acute diarrhea syndrome coronavirus nucleocapsid protein using a novel monoclonal antibody #MMPMID31518629 Han Y; Zhang J; Shi H; Zhou L; Chen J; Zhang X; Liu J; Zhang J; Wang X; Ji Z; Jing Z; Cong G; Ma J; Shi D; Li F Virus Res 2019[Nov]; 273 (ä): 197752 PMID31518629show ga A swine acute diarrhea syndrome coronavirus (SADS-CoV) that causes severe diarrhea in suckling piglets was identified in Southern China in 2017. To develop an antigen that is specific, sensitive, and easy to prepare for serological diagnosis, antigenic sites in the SADS-CoV nucleocapsid (N) protein were screened. We generated and characterized an N-reactive monoclonal antibody (mAb) 3E9 from mice immunized with recombinant N protein. Through fine epitope mapping of mAb 3E9 using a panel of eukaryotic-expressed polypeptides with GFP-tags, we identified the motif (343)DAPVFTPAP(351) as the minimal unit of the linear B-cell epitope recognized by mAb 3E9. Protein sequence alignment indicated that (343)DAPVFTPAP(351) was highly conserved in different SADS-CoV strains and SADS-related coronaviruses from bat, with one substitution in this motif in HKU2-related bat coronavirus. Using mAb 3E9, we observed that N protein was expressed in the cytoplasm and was in the nucleolus during SADS-CoV replication. N protein was immunoprecipitated from SADS-CoV-infected Vero E6 cells. Taken together, our results indicated that 3E9 mAb could be a useful tool to investigate the structure and function of N protein during viral replication. |*Epitope Mapping[MESH] |Alphacoronavirus/chemistry/*immunology[MESH] |Animals[MESH] |Animals, Suckling/virology[MESH] |Antibodies, Monoclonal/*immunology/isolation & purification[MESH] |Cell Nucleolus/*virology[MESH] |Chiroptera/virology[MESH] |Chlorocebus aethiops[MESH] |Coronavirus Infections/*veterinary/virology[MESH] |Coronavirus Nucleocapsid Proteins[MESH] |Cytoplasm/virology[MESH] |Diarrhea/veterinary/virology[MESH] |Female[MESH] |Mice[MESH] |Mice, Inbred BALB C[MESH] |Nucleocapsid Proteins/genetics/*immunology[MESH] |Swine[MESH] |Swine Diseases/immunology/virology[MESH] |Vero Cells[MESH]Epitope mapping and cellular localization of swine acute diarrhea synd(epmc).pdf DeepDyve Pubget Overpricing