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10.1002/jcb.29381 http://scihub22266oqcxt.onion/10.1002/jcb.29381 31512791!ä!31512791 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Cell+Biochem 2020 ; 121 (2): 1452-1462 Nephropedia Template TP {{tp|p=31512791|t=2020. Magnesium protects mouse hippocampal HT22 cells against hypoxia-induced injury by upregulation of miR-221 |pdf=|usr=}}{{31512791}} gab.com Text Magnesium protects mouse hippocampal HT22 cells against hypoxia-induced injury by upregulation of miR-221 JO: J Cell Biochem http://www.kidney.de/pget.php?&tw=1&pmid=31512791 #FOAvip Magnesium (Mg(2+) ) has been shown to exert neuroprotective effects against hypoxia. However, it still remains elusive whether Mg(2+) protected mouse hippocampal HT22 cells against hypoxia-evoked damages. Therefore, we aimed to investigate the function of Mg(2+) and mechanisms associated with microRNA-221 (miR-221). HT22 cells were exposed to 3% O(2) for 24 hours to induce hypoxic damages with 21% as a normoxic culture condition. The damages were monitored by viability, migration, and apoptosis of HT22 cells with or without Mg(2+) pretreatment. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was applied to examine the alteration of miR-221, miR-210, and miR-17-5p. Transduction was carried out to artificially alter the expression of miR-221 and nerve growth factor (NGF), which was confirmed by qRT-PCR or Western blot assays. To blunt phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) and nuclear factor kappaB (NF-kappaB), LY294002 (10 microM) and BAY 11-7082 (10 microM) were used. We observed Mg(2+) protected HT22 cells against hypoxia-induced damages by upregulating miR-221. Further, miR-221 positively regulated NGF expression. Overexpression of NGF alleviated cell injury, while suppression of NGF aggravated cell injury. Moreover, miR-221 elevated NGF by inducing phosphorylation of regulators in PI3K/AKT and NF-kappaB transduction cascades and then alleviated cell injury. In conclusion, Mg(2+) protected HT22 cells against hypoxia-induced damages by upregulation of miR-221 and NGF. These findings provided insights into the development of improved strategies for clinical application. Twit Text FOAVip Magnesium protects mouse hippocampal HT22 cells against hypoxia-induced injury by upregulation of miR-221 ????J Cell Biochem http://www.kidney.de/pget.php?&tw=1&pmid=31512791 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31512791 #FOAvip English Wikipedia <ref>{{Cite pmid|31512791}}</ref> Magnesium protects mouse hippocampal HT22 cells against hypoxia-induced injury by upregulation of miR-221 #MMPMID31512791 Mi F; Liu F; Zhang C J Cell Biochem 2020[Feb]; 121 (2): 1452-1462 PMID31512791show ga Magnesium (Mg(2+) ) has been shown to exert neuroprotective effects against hypoxia. However, it still remains elusive whether Mg(2+) protected mouse hippocampal HT22 cells against hypoxia-evoked damages. Therefore, we aimed to investigate the function of Mg(2+) and mechanisms associated with microRNA-221 (miR-221). HT22 cells were exposed to 3% O(2) for 24 hours to induce hypoxic damages with 21% as a normoxic culture condition. The damages were monitored by viability, migration, and apoptosis of HT22 cells with or without Mg(2+) pretreatment. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was applied to examine the alteration of miR-221, miR-210, and miR-17-5p. Transduction was carried out to artificially alter the expression of miR-221 and nerve growth factor (NGF), which was confirmed by qRT-PCR or Western blot assays. To blunt phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) and nuclear factor kappaB (NF-kappaB), LY294002 (10 microM) and BAY 11-7082 (10 microM) were used. We observed Mg(2+) protected HT22 cells against hypoxia-induced damages by upregulating miR-221. Further, miR-221 positively regulated NGF expression. Overexpression of NGF alleviated cell injury, while suppression of NGF aggravated cell injury. Moreover, miR-221 elevated NGF by inducing phosphorylation of regulators in PI3K/AKT and NF-kappaB transduction cascades and then alleviated cell injury. In conclusion, Mg(2+) protected HT22 cells against hypoxia-induced damages by upregulation of miR-221 and NGF. These findings provided insights into the development of improved strategies for clinical application. äMagnesium protects mouse hippocampal HT22 cells against hypoxia-induce(epmc).pdf DeepDyve Pubget Overpricing