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10.1007/s11262-019-01701-y http://scihub22266oqcxt.onion/10.1007/s11262-019-01701-y 31463771!7088929!31463771     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=31463771&cmd=llinks): Failed to open stream: HTTP request failed! 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Short hairpin RNAs targeting M and N genes reduce replication of porcine deltacoronavirus in ST cells |pdf=|usr=}}{{31463771}} gab.com Text Short hairpin RNAs targeting M and N genes reduce replication of porcine deltacoronavirus in ST cells JO: Virus Genes http://www.kidney.de/pget.php?&tw=1&pmid=31463771 #FOAvip Porcine deltacoronavirus (PDCoV) is a recently identified coronavirus that causes intestinal diseases in neonatal piglets with diarrhea, vomiting, dehydration, and post-infection mortality of 50-100%. Currently, there are no effective treatments or vaccines available to control PDCoV. To study the potential of RNA interference (RNAi) as a strategy against PDCoV infection, two short hairpin RNA (shRNA)-expressing plasmids (pGenesil-M and pGenesil-N) that targeted the M and N genes of PDCoV were constructed and transfected separately into swine testicular (ST) cells, which were then infected with PDCoV strain HB-BD. The potential of the plasmids to inhibit PDCoV replication was evaluated by cytopathic effect, virus titers, and real-time quantitative RT-PCR assay. The cytopathogenicity assays demonstrated that pGenesil-M and pGenesil-N protected ST cells against pathological changes with high specificity and efficacy. The 50% tissue culture infective dose showed that the PDCoV titers in ST cells treated with pGenesil-M and pGenesil-N were reduced 13.2- and 32.4-fold, respectively. Real-time quantitative RT-PCR also confirmed that the amount of viral RNA in cell cultures pre-transfected with pGenesil-M and pGenesil-N was reduced by 45.8 and 56.1%, respectively. This is believed to be the first report to show that shRNAs targeting the M and N genes of PDCoV exert antiviral effects in vitro, which suggests that RNAi is a promising new strategy against PDCoV infection. Twit Text FOAVip Short hairpin RNAs targeting M and N genes reduce replication of porcine deltacoronavirus in ST cells ????Virus Genes http://www.kidney.de/pget.php?&tw=1&pmid=31463771 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31463771 #FOAvip English Wikipedia <ref>{{Cite pmid|31463771}}</ref> Short hairpin RNAs targeting M and N genes reduce replication of porcine deltacoronavirus in ST cells #MMPMID31463771 Gu WY; Li Y; Liu BJ; Wang J; Yuan GF; Chen SJ; Zuo YZ; Fan JH Virus Genes 2019[Dec]; 55 (6): 795-801 PMID31463771show ga Porcine deltacoronavirus (PDCoV) is a recently identified coronavirus that causes intestinal diseases in neonatal piglets with diarrhea, vomiting, dehydration, and post-infection mortality of 50-100%. Currently, there are no effective treatments or vaccines available to control PDCoV. To study the potential of RNA interference (RNAi) as a strategy against PDCoV infection, two short hairpin RNA (shRNA)-expressing plasmids (pGenesil-M and pGenesil-N) that targeted the M and N genes of PDCoV were constructed and transfected separately into swine testicular (ST) cells, which were then infected with PDCoV strain HB-BD. The potential of the plasmids to inhibit PDCoV replication was evaluated by cytopathic effect, virus titers, and real-time quantitative RT-PCR assay. The cytopathogenicity assays demonstrated that pGenesil-M and pGenesil-N protected ST cells against pathological changes with high specificity and efficacy. The 50% tissue culture infective dose showed that the PDCoV titers in ST cells treated with pGenesil-M and pGenesil-N were reduced 13.2- and 32.4-fold, respectively. Real-time quantitative RT-PCR also confirmed that the amount of viral RNA in cell cultures pre-transfected with pGenesil-M and pGenesil-N was reduced by 45.8 and 56.1%, respectively. This is believed to be the first report to show that shRNAs targeting the M and N genes of PDCoV exert antiviral effects in vitro, which suggests that RNAi is a promising new strategy against PDCoV infection. |Animals[MESH] |Coronavirus Infections/*genetics/pathology/virology[MESH] |Coronavirus/*genetics/pathogenicity[MESH] |Diarrhea/genetics/pathology/veterinary/virology[MESH] |Male[MESH] |Plasmids/genetics[MESH] |RNA Interference[MESH] |RNA, Small Interfering/genetics[MESH] |RNA, Viral/genetics[MESH] |Swine Diseases/genetics/virology[MESH] |Swine/virology[MESH] |Testis/growth & development/virology[MESH] |Viral Proteins/*genetics[MESH]Short hairpin RNAs targeting M and N genes reduce replication of porci(epmc).pdf DeepDyve Pubget Overpricing