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Detecting amplicons of loop-mediated isothermal amplification #MMPMID31342547
Shirato K
Microbiol Immunol 2019[Oct]; 63 (10): 407-412 PMID31342547show ga
Loop-mediated isothermal amplification (LAMP) assays are used to detect diverse pathogens. Initially, LAMP amplicons were detected using electrophoresis; later, real-time monitoring based on turbidity was developed to overcome the problem of contamination with environmental DNA. Recently, real-time monitoring of fluorescence signals using a quenching primer and probe has improved the reliability of amplification signals. Here, methods of detecting LAMP amplicons are reviewed.