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10.1111/1348-0421.12734

http://scihub22266oqcxt.onion/10.1111/1348-0421.12734
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31342547!7168367!31342547
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suck abstract from ncbi


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pmid31342547      Microbiol+Immunol 2019 ; 63 (10): 407-412
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  • Detecting amplicons of loop-mediated isothermal amplification #MMPMID31342547
  • Shirato K
  • Microbiol Immunol 2019[Oct]; 63 (10): 407-412 PMID31342547show ga
  • Loop-mediated isothermal amplification (LAMP) assays are used to detect diverse pathogens. Initially, LAMP amplicons were detected using electrophoresis; later, real-time monitoring based on turbidity was developed to overcome the problem of contamination with environmental DNA. Recently, real-time monitoring of fluorescence signals using a quenching primer and probe has improved the reliability of amplification signals. Here, methods of detecting LAMP amplicons are reviewed.
  • |Bacterial Infections/*microbiology[MESH]
  • |DNA, Bacterial/*analysis[MESH]
  • |DNA, Protozoan/*analysis[MESH]
  • |DNA, Viral/*analysis[MESH]
  • |Fluorescence[MESH]
  • |Nephelometry and Turbidimetry/methods[MESH]
  • |Nucleic Acid Amplification Techniques/*methods[MESH]
  • |Protozoan Infections/*parasitology[MESH]
  • |Reproducibility of Results[MESH]


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