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10.1371/journal.pone.0217925 http://scihub22266oqcxt.onion/10.1371/journal.pone.0217925 31163064!6548430!31163064     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2019 ; 14 (6): e0217925 Nephropedia Template TP {{tp|p=31163064|t=2019. Low extracellular magnesium does not impair glucose-stimulated insulin secretion |pdf=|usr=}}{{31163064}} gab.com Text Low extracellular magnesium does not impair glucose-stimulated insulin secretion JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=31163064 #FOAvip There is an increasing amount of clinical evidence that hypomagnesemia (serum Mg2+ levels < 0.7 mmol/l) contributes to type 2 diabetes mellitus pathogenesis. Amongst other hypotheses, it has been suggested that Mg2+ deficiency affects insulin secretion. The aim of this study was, therefore, to investigate the acute effects of extracellular Mg2+ on glucose-stimulated insulin secretion in primary mouse islets of Langerhans and the rat insulinoma INS-1 cell line. Here we show that acute lowering of extracellular Mg2+ concentrations from 1.0 mM to 0.5 mM did not affect glucose-stimulated insulin secretion in islets or in insulin-secreting INS-1 cells. The expression of key genes in the insulin secretory pathway (e.g. Gck, Abcc8) was also unchanged in both experimental models. Knockdown of the most abundant Mg2+ channel Trpm7 by siRNAs in INS-1 cells resulted in a 3-fold increase in insulin secretion at stimulatory glucose conditions compared to mock-transfected cells. Our data suggest that insulin secretion is not affected by acute lowering of extracellular Mg2+ concentrations. Twit Text FOAVip Low extracellular magnesium does not impair glucose-stimulated insulin secretion ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=31163064 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31163064 #FOAvip English Wikipedia <ref>{{Cite pmid|31163064}}</ref> Low extracellular magnesium does not impair glucose-stimulated insulin secretion #MMPMID31163064 Gommers LMM; Hill TG; Ashcroft FM; de Baaij JHF PLoS One 2019[]; 14 (6): e0217925 PMID31163064show ga There is an increasing amount of clinical evidence that hypomagnesemia (serum Mg2+ levels < 0.7 mmol/l) contributes to type 2 diabetes mellitus pathogenesis. Amongst other hypotheses, it has been suggested that Mg2+ deficiency affects insulin secretion. The aim of this study was, therefore, to investigate the acute effects of extracellular Mg2+ on glucose-stimulated insulin secretion in primary mouse islets of Langerhans and the rat insulinoma INS-1 cell line. Here we show that acute lowering of extracellular Mg2+ concentrations from 1.0 mM to 0.5 mM did not affect glucose-stimulated insulin secretion in islets or in insulin-secreting INS-1 cells. The expression of key genes in the insulin secretory pathway (e.g. Gck, Abcc8) was also unchanged in both experimental models. Knockdown of the most abundant Mg2+ channel Trpm7 by siRNAs in INS-1 cells resulted in a 3-fold increase in insulin secretion at stimulatory glucose conditions compared to mock-transfected cells. Our data suggest that insulin secretion is not affected by acute lowering of extracellular Mg2+ concentrations. |Animals[MESH] |Extracellular Space/*chemistry[MESH] |Gene Expression Regulation/drug effects[MESH] |Glucose/*pharmacology[MESH] |Hyperglycemia/pathology[MESH] |Insulin Secretion/*drug effects[MESH] |Insulin-Secreting Cells/drug effects/metabolism/pathology[MESH] |Magnesium/*pharmacology[MESH] |Mice, Inbred C57BL[MESH] |RNA, Messenger/genetics/metabolism[MESH] |Rats[MESH]Low extracellular magnesium does not impair glucose-stimulated insulin(epmc).pdf DeepDyve Pubget Overpricing