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10.1016/j.bbrc.2019.03.211 http://scihub22266oqcxt.onion/10.1016/j.bbrc.2019.03.211 30967261!ä!30967261 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biochem+Biophys+Res+Commun 2019 ; 513 (2): 405-411 Nephropedia Template TP {{tp|p=30967261|t=2019. Obesity worsens the outcome of influenza virus infection associated with impaired type I interferon induction in mice |pdf=|usr=}}{{30967261}} gab.com Text Obesity worsens the outcome of influenza virus infection associated with impaired type I interferon induction in mice JO: Biochem Biophys Res Commun http://www.kidney.de/pget.php?&tw=1&pmid=30967261 #FOAvip Increasing evidence indicates that obesity is a risk factor for increased severity of influenza virus infection. However, its precise immunological mechanism is not fully understood. To investigate this, diet-induced obese (DIO) mice were established by feeding C57BL/6 male mice a high-fat diet for 16 weeks. DIO and lean control mice were infected intranasally with 3000?pfu of influenza A virus (IAV) (PR8/H1N1). Interestingly, we found adipose tissue located along the bronchus in naive DIO mice. In addition, the Nos2 level was significantly higher and Arg1 level was significantly lower in lung macrophages of naive DIO mice, consistent with an M1-skewed phenotype. The survival rate and body weight of DIO mice infected with IAV were significantly lower than those of lean control mice and associated with higher viral load in the lungs of DIO mice. Histopathological analysis demonstrated higher numbers of inflammatory cells in the lungs of DIO mice after IAV infection. Levels of cytokines, including TNF-alpha, IL-6, IL-10, and type I IFN (IFN-alpha and IFN-beta), in bronchoalveolar lavage fluid (BALF) were altered after IAV infection; in particular, IFN-alpha and IFN-beta levels were significantly suppressed in the BALF of DIO mice. In vitro, bone marrow-derived macrophages were stimulated with ligands of toll-like receptor (TLR) 7/8, a pattern recognition receptor for single-stranded RNA, and levels of TNF-alpha, IL-6, and IL-10 were similarly altered. In addition, levels of IFN-alpha and IFN-beta were significantly lower in culture supernatants of alveolar macrophages sorted from naive DIO mice and infected with IAV, compared to those in macrophages sorted from lean control mice. Collectively, these results suggest that macrophages may be the main contributors to poor outcomes of influenza virus infection in obesity. Twit Text FOAVip Obesity worsens the outcome of influenza virus infection associated with impaired type I interferon induction in mice ????Biochem Biophys Res Commun http://www.kidney.de/pget.php?&tw=1&pmid=30967261 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30967261 #FOAvip English Wikipedia <ref>{{Cite pmid|30967261}}</ref> Obesity worsens the outcome of influenza virus infection associated with impaired type I interferon induction in mice #MMPMID30967261 Namkoong H; Ishii M; Fujii H; Asami T; Yagi K; Suzuki S; Azekawa S; Tasaka S; Hasegawa N; Betsuyaku T Biochem Biophys Res Commun 2019[May]; 513 (2): 405-411 PMID30967261show ga Increasing evidence indicates that obesity is a risk factor for increased severity of influenza virus infection. However, its precise immunological mechanism is not fully understood. To investigate this, diet-induced obese (DIO) mice were established by feeding C57BL/6 male mice a high-fat diet for 16 weeks. DIO and lean control mice were infected intranasally with 3000?pfu of influenza A virus (IAV) (PR8/H1N1). Interestingly, we found adipose tissue located along the bronchus in naive DIO mice. In addition, the Nos2 level was significantly higher and Arg1 level was significantly lower in lung macrophages of naive DIO mice, consistent with an M1-skewed phenotype. The survival rate and body weight of DIO mice infected with IAV were significantly lower than those of lean control mice and associated with higher viral load in the lungs of DIO mice. Histopathological analysis demonstrated higher numbers of inflammatory cells in the lungs of DIO mice after IAV infection. Levels of cytokines, including TNF-alpha, IL-6, IL-10, and type I IFN (IFN-alpha and IFN-beta), in bronchoalveolar lavage fluid (BALF) were altered after IAV infection; in particular, IFN-alpha and IFN-beta levels were significantly suppressed in the BALF of DIO mice. In vitro, bone marrow-derived macrophages were stimulated with ligands of toll-like receptor (TLR) 7/8, a pattern recognition receptor for single-stranded RNA, and levels of TNF-alpha, IL-6, and IL-10 were similarly altered. In addition, levels of IFN-alpha and IFN-beta were significantly lower in culture supernatants of alveolar macrophages sorted from naive DIO mice and infected with IAV, compared to those in macrophages sorted from lean control mice. Collectively, these results suggest that macrophages may be the main contributors to poor outcomes of influenza virus infection in obesity. |Animals[MESH] |Bronchoalveolar Lavage Fluid/immunology/virology[MESH] |Disease Models, Animal[MESH] |Humans[MESH] |Influenza A Virus, H1N1 Subtype/*immunology[MESH] |Influenza, Human/complications/immunology/virology[MESH] |Interferon-alpha/*immunology[MESH] |Interferon-beta/*immunology[MESH] |Macrophages, Alveolar/immunology/virology[MESH] |Male[MESH] |Mice[MESH] |Mice, Inbred C57BL[MESH] |Obesity/*complications/immunology/virology[MESH]Obesity worsens the outcome of influenza virus infection associated wi(epmc).pdf DeepDyve Pubget Overpricing