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10.1099/0022-1317-67-2-235

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3080548!ä!3080548

suck abstract from ncbi


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pmid3080548      J+Gen+Virol 1986 ; 67 ( Pt 2) (ä): 235-41
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  • Purification and properties of an intranuclear virus-specific antigen from tissue infected with Borna disease virus #MMPMID3080548
  • Haas B; Becht H; Rott R
  • J Gen Virol 1986[Feb]; 67 ( Pt 2) (ä): 235-41 PMID3080548show ga
  • A virus-specific antigen was extracted from brains of rats and from MDCK cells infected with Borna disease (BD) virus and purified to homogeneity by immunoaffinity chromatography and HPLC. The antigen consists of two components which are almost equal in size (38 000 mol. wt.), and it forms aggregates in its native form. The virus specificity of the two antigenic entities was confirmed by immunoblots with convalescent serum and monoclonal antibodies. Immunofluorescent staining with monoclonal antibodies and a hyperimmune serum prepared against the purified antigen showed the intranuclear fluorescence typical for BD virus-infected cells.
  • |Animals[MESH]
  • |Antibodies, Monoclonal[MESH]
  • |Antigens, Viral/*isolation & purification[MESH]
  • |Borna disease virus/*immunology[MESH]
  • |Brain/microbiology[MESH]
  • |Cell Line[MESH]
  • |Cell Nucleus/*immunology/microbiology[MESH]
  • |Chromatography, Affinity[MESH]
  • |Chromatography, High Pressure Liquid[MESH]
  • |Dogs[MESH]
  • |Electrophoresis, Polyacrylamide Gel[MESH]
  • |Kidney[MESH]
  • |Mice[MESH]
  • |Mice, Inbred BALB C/immunology[MESH]
  • |Rats[MESH]
  • |Rats, Inbred Lew[MESH]


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