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10.1161/HYPERTENSIONAHA.118.11621 http://scihub22266oqcxt.onion/10.1161/HYPERTENSIONAHA.118.11621 30571558!6319266!30571558     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Hypertension 2019 ; 73 (1): 112-120 Nephropedia Template TP {{tp|p=30571558|t=2019. Norepinephrine-Induced Stimulation of Kir4 1/Kir5 1 Is Required for the Activation of NaCl Transporter in Distal Convoluted Tubule |pdf=|usr=}}{{30571558}} gab.com Text Norepinephrine-Induced Stimulation of Kir4 1/Kir5 1 Is Required for the Activation of NaCl Transporter in Distal Convoluted Tubule JO: Hypertension http://www.kidney.de/pget.php?&tw=1&pmid=30571558 #FOAvip The stimulation of beta-adrenergic receptor increases thiazide-sensitive NaCl cotransporter (NCC), an effect contributing to salt-sensitive hypertension by sympathetic stimulation. We now test whether the stimulation of beta-adrenergic receptor-induced activation of NCC is achieved through activating basolateral Kir4.1 in the distal convoluted tubule (DCT). Application of norepinephrine increased the basolateral 40 pS K(+) channel (Kir4.1/Kir5.1 heterotetramer) in the DCT. The stimulatory effect of norepinephrine on the K(+) channel was mimicked by cAMP analogue but abolished by inhibiting PKA (protein kinase A). Also, the effect of norepinephrine on the K(+) channel in the DCT was recapitulated by isoproterenol but not by alpha-adrenergic agonist and blocked by propranolol, suggesting that norepinephrine effect on the K(+) channel was mediated by beta-adrenergic receptor. The whole-cell recording shows that norepinephrine and isoproterenol increased DCT K(+) currents and shifted the K(+) current ( I(K)) reversal potential to negative range (hyperpolarization). Continuous norepinephrine perfusion (7 days) increased DCT K(+) currents, hyperpolarized I(K) reversal potential, and increased the expression of total NCC/phosphorylated NCC, but it had no significant effect on the expression of NKCC2 (type 2 Na-Cl-K cotransporter) and ENaC-alpha (epithelial Na channel-alpha subunit). Renal clearance study demonstrated that norepinephrine perfusion augmented thiazide-induced urinary Na(+) excretion only in wild-type but not in kidney-specific Kir4.1 knockout mice, suggesting that Kir4.1 is required for mediating the effect of norepinephrine on NCC. However, norepinephrine perfusion did not affect urinary K(+) excretion. We conclude that the stimulation of beta-adrenergic receptor activates the basolateral Kir4.1 in the DCT and that the activation of Kir4.1 is required for norepinephrine-induced stimulation of NCC. Twit Text FOAVip Norepinephrine-Induced Stimulation of Kir4 1/Kir5 1 Is Required for the Activation of NaCl Transporter in Distal Convoluted Tubule ????Hypertension http://www.kidney.de/pget.php?&tw=1&pmid=30571558 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30571558 #FOAvip English Wikipedia <ref>{{Cite pmid|30571558}}</ref> Norepinephrine-Induced Stimulation of Kir4 1/Kir5 1 Is Required for the Activation of NaCl Transporter in Distal Convoluted Tubule #MMPMID30571558 Duan XP; Gu L; Xiao Y; Gao ZX; Wu P; Zhang YH; Meng XX; Wang JL; Zhang DD; Lin DH; Wang WH; Gu R Hypertension 2019[Jan]; 73 (1): 112-120 PMID30571558show ga The stimulation of beta-adrenergic receptor increases thiazide-sensitive NaCl cotransporter (NCC), an effect contributing to salt-sensitive hypertension by sympathetic stimulation. We now test whether the stimulation of beta-adrenergic receptor-induced activation of NCC is achieved through activating basolateral Kir4.1 in the distal convoluted tubule (DCT). Application of norepinephrine increased the basolateral 40 pS K(+) channel (Kir4.1/Kir5.1 heterotetramer) in the DCT. The stimulatory effect of norepinephrine on the K(+) channel was mimicked by cAMP analogue but abolished by inhibiting PKA (protein kinase A). Also, the effect of norepinephrine on the K(+) channel in the DCT was recapitulated by isoproterenol but not by alpha-adrenergic agonist and blocked by propranolol, suggesting that norepinephrine effect on the K(+) channel was mediated by beta-adrenergic receptor. The whole-cell recording shows that norepinephrine and isoproterenol increased DCT K(+) currents and shifted the K(+) current ( I(K)) reversal potential to negative range (hyperpolarization). Continuous norepinephrine perfusion (7 days) increased DCT K(+) currents, hyperpolarized I(K) reversal potential, and increased the expression of total NCC/phosphorylated NCC, but it had no significant effect on the expression of NKCC2 (type 2 Na-Cl-K cotransporter) and ENaC-alpha (epithelial Na channel-alpha subunit). Renal clearance study demonstrated that norepinephrine perfusion augmented thiazide-induced urinary Na(+) excretion only in wild-type but not in kidney-specific Kir4.1 knockout mice, suggesting that Kir4.1 is required for mediating the effect of norepinephrine on NCC. However, norepinephrine perfusion did not affect urinary K(+) excretion. We conclude that the stimulation of beta-adrenergic receptor activates the basolateral Kir4.1 in the DCT and that the activation of Kir4.1 is required for norepinephrine-induced stimulation of NCC. |*Ion Transport/drug effects/physiology[MESH] |Adrenergic beta-Agonists/pharmacology[MESH] |Adrenergic beta-Antagonists/pharmacology[MESH] |Animals[MESH] |Isoproterenol/*pharmacology[MESH] |Kidney Tubules, Distal/drug effects/metabolism[MESH] |Kir5.1 Channel[MESH] |Mice[MESH] |Mice, Knockout[MESH] |Norepinephrine/metabolism[MESH] |Potassium Channels, Inwardly Rectifying/*metabolism[MESH] |Propranolol/*pharmacology[MESH] |Receptors, Adrenergic, beta/*metabolism[MESH] |Solute Carrier Family 12, Member 1/*metabolism[MESH]Norepinephrine-Induced Stimulation of Kir4 1/Kir5 1 Is Required for th(epmc).pdf DeepDyve Pubget Overpricing