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10.1042/BSR20180793 http://scihub22266oqcxt.onion/10.1042/BSR20180793 30538170!6328932!30538170     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosci+Rep 2019 ; 39 (1): ä Nephropedia Template TP {{tp|p=30538170|t=2019. Procaspase activating compound 1 controls tetracycline repressor-regulated gene expression system |pdf=|usr=}}{{30538170}} gab.com Text Procaspase activating compound 1 controls tetracycline repressor-regulated gene expression system JO: Biosci Rep http://www.kidney.de/pget.php?&tw=1&pmid=30538170 #FOAvip The tetracycline repressor (TetR)-regulated system is a widely used tool to study gene functions through control of its expression. Various effectors such as tetracycline (Tc) and doxycycline (Dox) quickly induce or shut down gene expression, but reversing gene expression has not been eligible due to long half-lives of such effectors. Here, we found that procaspase activating compound 1 (PAC-1) rapidly reduces transient expression of TetR-regulated green fluorescent protein (GFP) in mammalian cells. Next, we applied PAC-1 to control of expression of transient receptor potential melastatin 7 (TRPM7) protein, whose downstream cellular events can be monitored by cell morphological changes. We observed that PAC-1 quickly reduces TRPM7 expression, consequently affecting cell morphology regulated by TRPM7. The present study demonstrates the first small molecule that efficiently turns off the TetR-regulated gene expression in mammalian cells, thereby precisely regulating the expression level of target gene. Twit Text FOAVip Procaspase activating compound 1 controls tetracycline repressor-regulated gene expression system ????Biosci Rep http://www.kidney.de/pget.php?&tw=1&pmid=30538170 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30538170 #FOAvip English Wikipedia <ref>{{Cite pmid|30538170}}</ref> Procaspase activating compound 1 controls tetracycline repressor-regulated gene expression system #MMPMID30538170 Song C; Kim N; Park M; Lee J; Oh KB; Sim T Biosci Rep 2019[Jan]; 39 (1): ä PMID30538170show ga The tetracycline repressor (TetR)-regulated system is a widely used tool to study gene functions through control of its expression. Various effectors such as tetracycline (Tc) and doxycycline (Dox) quickly induce or shut down gene expression, but reversing gene expression has not been eligible due to long half-lives of such effectors. Here, we found that procaspase activating compound 1 (PAC-1) rapidly reduces transient expression of TetR-regulated green fluorescent protein (GFP) in mammalian cells. Next, we applied PAC-1 to control of expression of transient receptor potential melastatin 7 (TRPM7) protein, whose downstream cellular events can be monitored by cell morphological changes. We observed that PAC-1 quickly reduces TRPM7 expression, consequently affecting cell morphology regulated by TRPM7. The present study demonstrates the first small molecule that efficiently turns off the TetR-regulated gene expression in mammalian cells, thereby precisely regulating the expression level of target gene. |Gene Expression Regulation/*drug effects/genetics[MESH] |Gene Expression/drug effects/genetics[MESH] |Green Fluorescent Proteins/genetics[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Hydrazones/*pharmacology[MESH] |Piperazines/*pharmacology[MESH] |Repressor Proteins/*genetics[MESH] |TRPM Cation Channels/genetics[MESH]Procaspase activating compound 1 controls tetracycline repressor-regul(epmc).pdf DeepDyve Pubget Overpricing