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10.1002/jbm.b.34273 http://scihub22266oqcxt.onion/10.1002/jbm.b.34273 30508321!ä!30508321 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Biomed+Mater+Res+B+Appl+Biomater 2019 ; 107 (6): 1806-1813 Nephropedia Template TP {{tp|p=30508321|t=2019. Periprosthetic hypoxia as consequence of TRPM7 mediated cobalt influx in osteoblasts |pdf=|usr=}}{{30508321}} gab.com Text Periprosthetic hypoxia as consequence of TRPM7 mediated cobalt influx in osteoblasts JO: J Biomed Mater Res B Appl Biomater http://www.kidney.de/pget.php?&tw=1&pmid=30508321 #FOAvip The reasons for the high number of loosened metal-on-metal (MoM) hip implants are still not fully understood. Hypoxia-inducible factor 1 (HIF-1) mediated signaling pathways, which normally modulate tissue metabolism under hypoxic circumstances, could be triggered by metallic wear debris and influence bone metabolism favoring osteolysis. This may lead to early loosening of the orthopedic implants. Immunhistochemical staining of periprosthetic tissues of failed artificial hip implants showed that the concentration of HIF-1alpha in the surrounding tissues of failed MoM hip implants was significantly higher in comparison to failed metal-on-polyethylene (MoP) hip implants and osteoarthritic tissues. Therefore, we examined the Co(2+) -uptake mechanisms and the influence of Co(2+) uptake on HIF-1alpha stabilization. Based on cobalt mediated quenching effects, calcium imaging experiments using fura-2 showed a concentration-dependent cobalt influx in MG-63 cells, which could be inhibited by the unspecific TRPM7 channel inhibitor 2-APB (20 muM) and TRPM7 specific siRNA. Western blots confirmed a dose dependent increase of HIF-1alpha upon stimulation with Co(2+) . This effect could be abrogated by inhibition of cobalt influx using 2-APB. This study shows that chemical hypoxia originating from HIF-1alpha upregulation within the periprosthetic tissue is related to cobalt wear debris and highlights TRPM7 as an important key mediator in this context. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1806-1813, 2019. Twit Text FOAVip Periprosthetic hypoxia as consequence of TRPM7 mediated cobalt influx in osteoblasts ????J Biomed Mater Res B Appl Biomater http://www.kidney.de/pget.php?&tw=1&pmid=30508321 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30508321 #FOAvip English Wikipedia <ref>{{Cite pmid|30508321}}</ref> Periprosthetic hypoxia as consequence of TRPM7 mediated cobalt influx in osteoblasts #MMPMID30508321 Rommelt C; Munsch T; Drynda A; Lessmann V; Lohmann CH; Bertrand J J Biomed Mater Res B Appl Biomater 2019[Aug]; 107 (6): 1806-1813 PMID30508321show ga The reasons for the high number of loosened metal-on-metal (MoM) hip implants are still not fully understood. Hypoxia-inducible factor 1 (HIF-1) mediated signaling pathways, which normally modulate tissue metabolism under hypoxic circumstances, could be triggered by metallic wear debris and influence bone metabolism favoring osteolysis. This may lead to early loosening of the orthopedic implants. Immunhistochemical staining of periprosthetic tissues of failed artificial hip implants showed that the concentration of HIF-1alpha in the surrounding tissues of failed MoM hip implants was significantly higher in comparison to failed metal-on-polyethylene (MoP) hip implants and osteoarthritic tissues. Therefore, we examined the Co(2+) -uptake mechanisms and the influence of Co(2+) uptake on HIF-1alpha stabilization. Based on cobalt mediated quenching effects, calcium imaging experiments using fura-2 showed a concentration-dependent cobalt influx in MG-63 cells, which could be inhibited by the unspecific TRPM7 channel inhibitor 2-APB (20 muM) and TRPM7 specific siRNA. Western blots confirmed a dose dependent increase of HIF-1alpha upon stimulation with Co(2+) . This effect could be abrogated by inhibition of cobalt influx using 2-APB. This study shows that chemical hypoxia originating from HIF-1alpha upregulation within the periprosthetic tissue is related to cobalt wear debris and highlights TRPM7 as an important key mediator in this context. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1806-1813, 2019. |*Gene Expression Regulation[MESH] |*Hip Prosthesis[MESH] |*Metal-on-Metal Joint Prostheses[MESH] |Cell Hypoxia[MESH] |Cell Line[MESH] |Cobalt/*pharmacokinetics[MESH] |Humans[MESH] |Hypoxia-Inducible Factor 1, alpha Subunit/biosynthesis[MESH] |Osteoblasts/*metabolism[MESH] |Protein Serine-Threonine Kinases/*biosynthesis[MESH]Periprosthetic hypoxia as consequence of TRPM7 mediated cobalt influx (epmc).pdf DeepDyve Pubget Overpricing