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10.1111/1348-0421.12643 http://scihub22266oqcxt.onion/10.1111/1348-0421.12643 30117617!7168444!30117617     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Microbiol+Immunol 2018 ; 62 (9): 574-584 Nephropedia Template TP {{tp|p=30117617|t=2018. Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus |pdf=|usr=}}{{30117617}} gab.com Text Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus JO: Microbiol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=30117617 #FOAvip Middle East respiratory syndrome coronavirus (MERS-CoV) is a single-stranded RNA virus that causes severe respiratory disease in humans with a high fatality rate. Binding of the receptor binding domain (RBD) of the spike (S) glycoprotein to dipeptidyl peptidase 4 is the critical step in MERS-CoV infection of a host cell. No vaccines or clinically applicable treatments are currently available for MERS-CoV. Therefore, rapid diagnosis is important for improving patient outcomes through prompt treatment and protection against viral outbreaks. In this study, the aim was to establish two ELISA systems for detecting antigens and antibodies against MERS-CoV. Using a recombinant full-length S protein, an indirect ELISA was developed and found to detect MERS-CoV-specific antibodies in animal sera and sera of patient with MERS. Moreover, MAbs were induced with the recombinant S protein and RBD and used for sandwich ELISA to detect the MERS-CoV S protein. Neither ELISA system exhibited significant intra-assay or inter-assay variation, indicating good reproducibility. Moreover, the inter-day precision and sensitivity were adequate for use as a diagnostic kit. Thus, these ELISAs can be used clinically to diagnose MERS-CoV. Twit Text FOAVip Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus ????Microbiol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=30117617 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30117617 #FOAvip English Wikipedia <ref>{{Cite pmid|30117617}}</ref> Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus #MMPMID30117617 Lee K; Ko HL; Lee EY; Park HJ; Kim YS; Kim YS; Cho NH; Park MS; Lee SM; Kim J; Kim H; Seong BL; Nam JH Microbiol Immunol 2018[Sep]; 62 (9): 574-584 PMID30117617show ga Middle East respiratory syndrome coronavirus (MERS-CoV) is a single-stranded RNA virus that causes severe respiratory disease in humans with a high fatality rate. Binding of the receptor binding domain (RBD) of the spike (S) glycoprotein to dipeptidyl peptidase 4 is the critical step in MERS-CoV infection of a host cell. No vaccines or clinically applicable treatments are currently available for MERS-CoV. Therefore, rapid diagnosis is important for improving patient outcomes through prompt treatment and protection against viral outbreaks. In this study, the aim was to establish two ELISA systems for detecting antigens and antibodies against MERS-CoV. Using a recombinant full-length S protein, an indirect ELISA was developed and found to detect MERS-CoV-specific antibodies in animal sera and sera of patient with MERS. Moreover, MAbs were induced with the recombinant S protein and RBD and used for sandwich ELISA to detect the MERS-CoV S protein. Neither ELISA system exhibited significant intra-assay or inter-assay variation, indicating good reproducibility. Moreover, the inter-day precision and sensitivity were adequate for use as a diagnostic kit. Thus, these ELISAs can be used clinically to diagnose MERS-CoV. |Animals[MESH] |Antibodies, Monoclonal[MESH] |Antibodies, Viral/blood/immunology/*isolation & purification[MESH] |Antigens, Viral/immunology/*isolation & purification[MESH] |Baculoviridae/genetics[MESH] |Coronavirus Infections/*diagnosis/*immunology/virology[MESH] |Enzyme-Linked Immunosorbent Assay/methods[MESH] |Escherichia coli/genetics[MESH] |Gene Expression[MESH] |Humans[MESH] |Immunologic Tests/*methods[MESH] |Middle East Respiratory Syndrome Coronavirus/genetics/*immunology[MESH] |Protein Binding/genetics/immunology[MESH] |Protein Interaction Domains and Motifs[MESH] |Rats[MESH] |Rats, Wistar[MESH] |Receptors, Virus/immunology[MESH] |Recombinant Proteins/genetics/immunology[MESH] |Reproducibility of Results[MESH] |Sensitivity and Specificity[MESH] |Sf9 Cells[MESH] |Spike Glycoprotein, Coronavirus/genetics/*immunology[MESH]Development of a diagnostic system for detection of specific antibodie(epmc).pdf DeepDyve Pubget Overpricing