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10.21769/BioProtoc.2926 http://scihub22266oqcxt.onion/10.21769/BioProtoc.2926 30112425!6089531!30112425     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=30112425&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Bio+Protoc 2018 ; 8 (14): ? Nephropedia Template TP {{tp|p=30112425|t=2018. Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry |pdf=|usr=}}{{30112425}} gab.com Text Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry JO: Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=30112425 #FOAvip After recognizing extracellular bacterial lipopolysaccharide (LPS), the toll-like receptor 4 (TLR4)-CD14 signaling complex initiates two distinct signaling pathways-one from the plasma membrane and the other from the signaling endosomes (Kagan et al., 2008). Understanding the early stages of TLR4 signal transduction therefore requires a robust and quantitative method to measure LPS-triggered TLR4 and CD14 receptor endocytosis, one of the earliest events of LPS detection. Here, we describe a flow cytometry-based method that we used recently to study the role of the ion channel TRPM7 in TLR4 endocytosis (Schappe et al., 2018). The assay relies on stimulating the cells with LPS and measuring the cell surface levels of TLR4 (or CD14) at various time points using flow cytometry. Although we detail the method specifically for TLR4 and CD14 from murine bone marrow-derived macrophages, it can be readily adapted to evaluate receptor endocytosis in a variety of other signaling contexts. Twit Text FOAVip Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry ????Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=30112425 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30112425 #FOAvip English Wikipedia <ref>{{Cite pmid|30112425}}</ref> Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry #MMPMID30112425 Schappe MS; Desai BN Bio Protoc 2018[Jul]; 8 (14): ? PMID30112425show ga After recognizing extracellular bacterial lipopolysaccharide (LPS), the toll-like receptor 4 (TLR4)-CD14 signaling complex initiates two distinct signaling pathways-one from the plasma membrane and the other from the signaling endosomes (Kagan et al., 2008). Understanding the early stages of TLR4 signal transduction therefore requires a robust and quantitative method to measure LPS-triggered TLR4 and CD14 receptor endocytosis, one of the earliest events of LPS detection. Here, we describe a flow cytometry-based method that we used recently to study the role of the ion channel TRPM7 in TLR4 endocytosis (Schappe et al., 2018). The assay relies on stimulating the cells with LPS and measuring the cell surface levels of TLR4 (or CD14) at various time points using flow cytometry. Although we detail the method specifically for TLR4 and CD14 from murine bone marrow-derived macrophages, it can be readily adapted to evaluate receptor endocytosis in a variety of other signaling contexts. ?Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry(epmc).pdf DeepDyve Pubget Overpricing