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10.1002/jmv.25263 http://scihub22266oqcxt.onion/10.1002/jmv.25263 30011073!ä!30011073 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2018 ; 90 (12): 1814-1821 Nephropedia Template TP {{tp|p=30011073|t=2018. Identification of potential pathogenic viruses in patients with acute myocarditis using next-generation sequencing |pdf=|usr=}}{{30011073}} gab.com Text Identification of potential pathogenic viruses in patients with acute myocarditis using next-generation sequencing JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=30011073 #FOAvip Myocarditis is an inflammatory disease of the myocardium and leads to cardiac dysfunction and heart failure. Although viral infections are considered to be the most common etiology of myocarditis, the identification of the causative virus is still challenging. Recently, next-generation sequencing (NGS) has been applied in the diagnosis of infectious diseases. The aim of the current study was to comprehensively analyze potential pathogenic microorganisms using NGS in the sera of patients with myocarditis. Twelve pediatric and five adult patients hospitalized for acute myocarditis were included. Serum samples in the acute phase were obtained and analyzed using NGS to detect pathogen-derived DNA and RNA. Viral sequence reads were detected in 7 (41%) of the 17 myocarditis patients by NGS. Among these patients, detection of Epstein-Barr virus, human parvovirus B19, torque teno virus, and respiratory syncytial virus reads by NGS was consistent with polymerase chain reaction or antigen test results in one patient each. A large number of human pegivirus reads were detected from one patient by RNA sequencing; however, its pathogenicity to human is unknown. Conversely, the number of detected virus-derived reads was small in most cases, and the pathophysiological role of these viruses remains to be clarified. No significant bacterial or fungal reads other than normal bacterial flora was detected. These data indicate that comprehensive detection of virus-derived DNA and RNA using NGS can be useful for the identification of potential pathogenic viruses in myocarditis. Twit Text FOAVip Identification of potential pathogenic viruses in patients with acute myocarditis using next-generation sequencing ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=30011073 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=30011073 #FOAvip English Wikipedia <ref>{{Cite pmid|30011073}}</ref> Identification of potential pathogenic viruses in patients with acute myocarditis using next-generation sequencing #MMPMID30011073 Takeuchi S; Kawada JI; Okuno Y; Horiba K; Suzuki T; Torii Y; Yasuda K; Numaguchi A; Kato T; Takahashi Y; Ito Y J Med Virol 2018[Dec]; 90 (12): 1814-1821 PMID30011073show ga Myocarditis is an inflammatory disease of the myocardium and leads to cardiac dysfunction and heart failure. Although viral infections are considered to be the most common etiology of myocarditis, the identification of the causative virus is still challenging. Recently, next-generation sequencing (NGS) has been applied in the diagnosis of infectious diseases. The aim of the current study was to comprehensively analyze potential pathogenic microorganisms using NGS in the sera of patients with myocarditis. Twelve pediatric and five adult patients hospitalized for acute myocarditis were included. Serum samples in the acute phase were obtained and analyzed using NGS to detect pathogen-derived DNA and RNA. Viral sequence reads were detected in 7 (41%) of the 17 myocarditis patients by NGS. Among these patients, detection of Epstein-Barr virus, human parvovirus B19, torque teno virus, and respiratory syncytial virus reads by NGS was consistent with polymerase chain reaction or antigen test results in one patient each. A large number of human pegivirus reads were detected from one patient by RNA sequencing; however, its pathogenicity to human is unknown. Conversely, the number of detected virus-derived reads was small in most cases, and the pathophysiological role of these viruses remains to be clarified. No significant bacterial or fungal reads other than normal bacterial flora was detected. These data indicate that comprehensive detection of virus-derived DNA and RNA using NGS can be useful for the identification of potential pathogenic viruses in myocarditis. |Adolescent[MESH] |Adult[MESH] |Aged[MESH] |Child[MESH] |Child, Preschool[MESH] |Female[MESH] |High-Throughput Nucleotide Sequencing/*methods[MESH] |Humans[MESH] |Infant[MESH] |Male[MESH] |Middle Aged[MESH] |Molecular Diagnostic Techniques/*methods[MESH] |Myocarditis/*virology[MESH] |Sensitivity and Specificity[MESH] |Serum/*virology[MESH] |Virus Diseases/*virology[MESH]Identification of potential pathogenic viruses in patients with acute (epmc).pdf DeepDyve Pubget Overpricing