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10.1007/s12576-018-0625-5 http://scihub22266oqcxt.onion/10.1007/s12576-018-0625-5 29909547!10717743!29909547     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Physiol+Sci 2019 ; 69 (1): 97-102 Nephropedia Template TP {{tp|p=29909547|t=2019. Modulation of Mg(2+) influx and cytoplasmic free Mg(2+) concentration in rat ventricular myocytes |pdf=|usr=}}{{29909547}} gab.com Text Modulation of Mg(2+) influx and cytoplasmic free Mg(2+) concentration in rat ventricular myocytes JO: J Physiol Sci http://www.kidney.de/pget.php?&tw=1&pmid=29909547 #FOAvip To examine whether TRPM7, a member of the melastatin family of transient receptor potential channels, is a physiological pathway for Mg(2+) entry in mammalian cells, we studied the effect of TRPM7 regulators on cytoplasmic free Mg(2+) concentration ([Mg(2+)](i)) of rat ventricular myocytes. Acutely isolated single cells were AM-loaded with the fluorescent indicator furaptra, and [Mg(2+)](i) was estimated at 25 degrees C. After [Mg(2+)](i) was lowered by soaking the cells with a high-K(+) and Mg(2+)-Ca(2+)-free solution, [Mg(2+)](i) was recovered by extracellular perfusion of Ca(2+)-free Tyrode's solution that contained 1 mM Mg(2+). The initial rate of increase in [Mg(2+)](i) was analyzed as the Mg(2+) influx rate. The Mg(2+) influx rate was increased by the TRPM7 activator, naltriben (2-50 muM), in a concentration-dependent manner with a half maximal effective concentration (EC(50)) of 24 muM. This EC(50) value is similar to that reported for the activation of recombinant TRPM7 overexpressed in HEK293 cells. Naltriben (50 muM) caused little change in basal [Mg(2+)](i) (~ 0.9 mM) in Ca(2+)-free Tyrode's solution, but significantly raised [Mg(2+)](i) to 1.31 +/- 0.03 mM in 94 min after the removal of extracellular Na(+). Re-introduction of extracellular Na(+) lowered [Mg(2+)](i) back to the basal level even in the presence of naltriben. Application of 10 muM NS8593, an inhibitor of TRPM7, significantly lowered [Mg(2+)](i) to 0.72 +/- 0.03 mM in 50-60 min independent of extracellular Na(+). The results suggest that Mg(2+) entry through TRPM7 significantly contributes to physiological Mg(2+) homeostasis in mammalian heart cells. Twit Text FOAVip Modulation of Mg(2+) influx and cytoplasmic free Mg(2+) concentration in rat ventricular myocytes ????J Physiol Sci http://www.kidney.de/pget.php?&tw=1&pmid=29909547 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29909547 #FOAvip English Wikipedia <ref>{{Cite pmid|29909547}}</ref> Modulation of Mg(2+) influx and cytoplasmic free Mg(2+) concentration in rat ventricular myocytes #MMPMID29909547 Tashiro M; Inoue H; Konishi M J Physiol Sci 2019[Jan]; 69 (1): 97-102 PMID29909547show ga To examine whether TRPM7, a member of the melastatin family of transient receptor potential channels, is a physiological pathway for Mg(2+) entry in mammalian cells, we studied the effect of TRPM7 regulators on cytoplasmic free Mg(2+) concentration ([Mg(2+)](i)) of rat ventricular myocytes. Acutely isolated single cells were AM-loaded with the fluorescent indicator furaptra, and [Mg(2+)](i) was estimated at 25 degrees C. After [Mg(2+)](i) was lowered by soaking the cells with a high-K(+) and Mg(2+)-Ca(2+)-free solution, [Mg(2+)](i) was recovered by extracellular perfusion of Ca(2+)-free Tyrode's solution that contained 1 mM Mg(2+). The initial rate of increase in [Mg(2+)](i) was analyzed as the Mg(2+) influx rate. The Mg(2+) influx rate was increased by the TRPM7 activator, naltriben (2-50 muM), in a concentration-dependent manner with a half maximal effective concentration (EC(50)) of 24 muM. This EC(50) value is similar to that reported for the activation of recombinant TRPM7 overexpressed in HEK293 cells. Naltriben (50 muM) caused little change in basal [Mg(2+)](i) (~ 0.9 mM) in Ca(2+)-free Tyrode's solution, but significantly raised [Mg(2+)](i) to 1.31 +/- 0.03 mM in 94 min after the removal of extracellular Na(+). Re-introduction of extracellular Na(+) lowered [Mg(2+)](i) back to the basal level even in the presence of naltriben. Application of 10 muM NS8593, an inhibitor of TRPM7, significantly lowered [Mg(2+)](i) to 0.72 +/- 0.03 mM in 50-60 min independent of extracellular Na(+). The results suggest that Mg(2+) entry through TRPM7 significantly contributes to physiological Mg(2+) homeostasis in mammalian heart cells. |1-Naphthylamine/analogs & derivatives/pharmacology[MESH] |Animals[MESH] |Calcium/metabolism[MESH] |Cell Line[MESH] |Cytoplasm/drug effects/*metabolism[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Magnesium/*metabolism[MESH] |Male[MESH] |Myocytes, Cardiac/drug effects/*metabolism[MESH] |Naltrexone/analogs & derivatives/pharmacology[MESH] |Rats[MESH] |Rats, Wistar[MESH]Modulation of Mg(2+) influx and cytoplasmic free Mg(2+) concentration (epmc).pdf DeepDyve Pubget Overpricing