The ion channel TRPM7 is required for B cell lymphopoiesis #MMPMID29871911
Krishnamoorthy M; Buhari FHM; Zhao T; Brauer PM; Burrows K; Cao EY; Moxley-Paquette V; Mortha A; Zuniga-Pflucker JC; Treanor B
Sci Signal 2018[Jun]; 11 (533): ? PMID29871911show ga
The transient receptor potential (TRP) family is a large family of widely expressed ion channels that regulate the intracellular concentration of ions and metals and respond to various chemical and physical stimuli. TRP subfamily M member 7 (TRPM7) is unusual in that it contains both an ion channel and a kinase domain. TRPM7 is a divalent cation channel with preference for Ca(2+) and Mg(2+) It is required for the survival of DT40 cells, a B cell line; however, deletion of TRPM7 in T cells does not impair their development. We found that expression of TRPM7 was required for B cell development in mice. Mice that lacked TRPM7 in B cells failed to generate peripheral B cells because of a developmental block at the pro-B cell stage. The loss of TRPM7 kinase activity alone did not affect the proportion of peripheral mature B cells or the development of B cells in the bone marrow. However, supplementation with a high concentration of extracellular Mg(2+) partially rescued the development of TRPM7-deficient B cells in vitro. Thus, our findings identify a critical role for TRPM7 ion channel activity in B cell development.
Sci+Signal 2018 ; 11 (533): ?
