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10.1002/bit.26734 http://scihub22266oqcxt.onion/10.1002/bit.26734 29777597!7161830!29777597     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biotechnol+Bioeng 2018 ; 115 (9): 2194-2204 Nephropedia Template TP {{tp|p=29777597|t=2018. Microdevice-based solid-phase polymerase chain reaction for rapid detection of pathogenic microorganisms |pdf=|usr=}}{{29777597}} gab.com Text Microdevice-based solid-phase polymerase chain reaction for rapid detection of pathogenic microorganisms JO: Biotechnol Bioeng http://www.kidney.de/pget.php?&tw=1&pmid=29777597 #FOAvip We demonstrate the integration of DNA amplification and detection functionalities developed on a lab-on-a-chip microdevice utilizing solid-phase polymerase chain reaction (SP-PCR) for point-of-need (PON) DNA analyses. First, the polycarbonate microdevice was fabricated by thermal bonding? to contain microchambers as reservoirs for performing SP-PCR. Next, the microchambers were subsequently modified with polyethyleneimine and glutaraldehyde for immobilizing amine-modified forward primers. During SP-PCR, the immobilized forward primers and freely diffusing fluorescence-labeled reverse primers cooperated to generate target amplicons, which remained covalently attached to the microchambers for the fluorescence detection. The SP-PCR microdevice was used for the direct identifications of two widely detected foodborne pathogens, namely Salmonella spp. and Staphylococcus aureus, and an alga causing harmful algal blooms annually in South Korea, Cochlodinium polykrikoides. The SP-PCR microdevice would be versatilely applied in PON testing as a universal platform for the fast identification of foodborne pathogens and environmentally threatening biogenic targets. Twit Text FOAVip Microdevice-based solid-phase polymerase chain reaction for rapid detection of pathogenic microorganisms ????Biotechnol Bioeng http://www.kidney.de/pget.php?&tw=1&pmid=29777597 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29777597 #FOAvip English Wikipedia <ref>{{Cite pmid|29777597}}</ref> Microdevice-based solid-phase polymerase chain reaction for rapid detection of pathogenic microorganisms #MMPMID29777597 Pham QN; Trinh KTL; Jung SW; Lee NY Biotechnol Bioeng 2018[Sep]; 115 (9): 2194-2204 PMID29777597show ga We demonstrate the integration of DNA amplification and detection functionalities developed on a lab-on-a-chip microdevice utilizing solid-phase polymerase chain reaction (SP-PCR) for point-of-need (PON) DNA analyses. First, the polycarbonate microdevice was fabricated by thermal bonding? to contain microchambers as reservoirs for performing SP-PCR. Next, the microchambers were subsequently modified with polyethyleneimine and glutaraldehyde for immobilizing amine-modified forward primers. During SP-PCR, the immobilized forward primers and freely diffusing fluorescence-labeled reverse primers cooperated to generate target amplicons, which remained covalently attached to the microchambers for the fluorescence detection. The SP-PCR microdevice was used for the direct identifications of two widely detected foodborne pathogens, namely Salmonella spp. and Staphylococcus aureus, and an alga causing harmful algal blooms annually in South Korea, Cochlodinium polykrikoides. The SP-PCR microdevice would be versatilely applied in PON testing as a universal platform for the fast identification of foodborne pathogens and environmentally threatening biogenic targets. |*Lab-On-A-Chip Devices[MESH] |Dinoflagellida/isolation & purification[MESH] |Microbiological Techniques/instrumentation/*methods[MESH] |Molecular Diagnostic Techniques/instrumentation/*methods[MESH] |Point-of-Care Systems[MESH] |Polymerase Chain Reaction/instrumentation/*methods[MESH] |Salmonella/isolation & purification[MESH] |Staphylococcus aureus/isolation & purification[MESH]Microdevice-based solid-phase polymerase chain reaction for rapid dete(epmc).pdf DeepDyve Pubget Overpricing