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Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 J+Nutr+Biochem 2018 ; 55 (ä): 200-208 Nephropedia Template TP
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An insight into the role of magnesium in the immunomodulatory properties of mesenchymal stem cells #MMPMID29554498
da Silva Lima F; da Rocha Romero AB; Hastreiter A; Nogueira-Pedro A; Makiyama E; Colli C; Fock RA
J Nutr Biochem 2018[May]; 55 (ä): 200-208 PMID29554498show ga
Magnesium (Mg(2+)) is a mineral with the ability to influence cell proliferation and to modulate inflammatory/immune responses, due to its anti-inflammatory properties. In addition, mesenchymal stem cells (MSCs) modulate the function of all major immune cell populations. Knowing that, the current work aimed to investigate the effects of Mg(2+) enrichment, and its influence on the immunomodulatory capacity of MSCs. Murine C3H/10T1/2 MSCs were cultivated in media with different concentrations of Mg(2+) (0, 1, 3 and 5 mM), in order to evaluate the effects of Mg(2+) on MSC immunomodulatory properties, cell proliferation rates, expression of NFkappaB and STAT-3, production of IL-1beta, IL-6, TGF-beta, IL-10, PGE2 and NO, and TRPM7 expression. The results showed that TRPM7 is expressed in MSCs, but Mg(2+), in the way that cells were cultivated, did not affect TRPM7 expression. Additionally, there was no difference in the intracellular concentration of Mg(2+). Mg(2+), especially at 5 mM, raised proliferation rates of MSCs, and modulated immune responses by decreasing levels of IL-1beta and IL-6, and by increasing levels of IL-10 and PGE2 in cells stimulated with LPS or TNF-alpha. In addition, MSCs cultured in 5 mM Mg(2+) expressed lower levels of pNFkappaB/NFkappaB and higher levels of pSTAT-3/STAT-3. Furthermore, conditioned media from MSCs reduced lymphocyte and macrophage proliferation, but Mg(2+) did not affect this parameter. In addition, conditioned media from MSCs cultured at 5 mM of Mg(2+) modulated the production profile of cytokines, especially of IL-1beta and IL-6 in macrophages. In conclusion, Mg(2+) is able to modulate some immunoregulatory properties of MSCs.
J+Nutr+Biochem 2018 ; 55 (ä): 200-208
