Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Biochem+J 2018 ; 475 (6): 1129-1139 Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Phosphatase of regenerating liver maintains cellular magnesium homeostasis #MMPMID29487165
Yoshida A; Funato Y; Miki H
Biochem J 2018[Mar]; 475 (6): 1129-1139 PMID29487165show ga
Phosphatase of regenerating liver (PRL) is highly expressed in malignant cancers and promotes cancer progression. Recent studies have suggested its functional relationship with Mg(2+), but the importance and molecular details of this relationship remain unknown. Here, we report that PRL expression is regulated by Mg(2+) and PRL protects cells from apoptosis under Mg(2+)-depleted conditions. When cultured cells were subjected to Mg(2+) depletion, endogenous PRL protein levels increased significantly. siRNA-mediated knockdown of endogenous PRL did not significantly affect cell proliferation under normal culture conditions, but it increased cell death after Mg(2+) depletion. Imaging analyses with a fluorescent probe for Mg(2+) showed that PRL knockdown severely reduced intracellular Mg(2+) levels, indicating a role for PRL in maintaining intracellular Mg(2+) We also examined the mechanism of augmented expression of PRL proteins and found that PRL mRNA transcription was stimulated by Mg(2+) depletion. A series of analyses revealed the activation and the crucial importance of signal transducer and activator of transcription 1 in this process. Collectively, these results implicate PRL in maintaining cellular Mg(2+) homeostasis.
Biochem+J 2018 ; 475 (6): 1129-1139
