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10.1016/j.antiviral.2017.08.005 http://scihub22266oqcxt.onion/10.1016/j.antiviral.2017.08.005 28807685/?report=reader!5654650!28807685     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Antiviral+Res 2017 ; 146 (ä): 21-27 Nephropedia Template TP {{tp|p=28807685|t=2017. An RNA polymerase II-driven Ebola virus minigenome system as an advanced tool for antiviral drug screening |pdf=|usr=}}{{28807685}} gab.com Text An RNA polymerase II-driven Ebola virus minigenome system as an advanced tool for antiviral drug screening JO: Antiviral Res http://www.kidney.de/pget.php?&tw=1&pmid=28807685 #FOAvip Ebola virus (EBOV) causes a severe disease in humans with the potential for significant international public health consequences. Currently, treatments are limited to experimental vaccines and therapeutics. Therefore, research into prophylaxis and antiviral strategies to combat EBOV infections is of utmost importance. The requirement for high containment laboratories to study EBOV infection is a limiting factor for conducting EBOV research. To overcome this issue, minigenome systems have been used as valuable tools to study EBOV replication and transcription mechanisms and to screen for antiviral compounds at biosafety level 2. The most commonly used EBOV minigenome system relies on the ectopic expression of the T7 RNA polymerase (T7), which can be limiting for certain cell types. We have established an improved EBOV minigenome system that utilizes endogenous RNA polymerase II (pol II) as a driver for the synthesis of minigenome RNA. We show here that this system is as efficient as the T7-based minigenome system, but works in a wider range of cell types, including biologically relevant cell types such as bat cells. Importantly, we were also able to adapt this system to a reliable and cost-effective 96-well format antiviral screening assay with a Z-factor of 0.74, indicative of a robust assay. Using this format, we identified JG40, an inhibitor of Hsp70, as an inhibitor of EBOV replication, highlighting the potential for this system as a tool for antiviral drug screening. In summary, this updated EBOV minigenome system provides a convenient and effective means of advancing the field of EBOV research. Twit Text FOAVip An RNA polymerase II-driven Ebola virus minigenome system as an advanced tool for antiviral drug screening ????Antiviral Res http://www.kidney.de/pget.php?&tw=1&pmid=28807685 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28807685 #FOAvip English Wikipedia <ref>{{Cite pmid|28807685}}</ref> An RNA polymerase II-driven Ebola virus minigenome system as an advanced tool for antiviral drug screening #MMPMID28807685 Nelson EV; Pacheco JR; Hume AJ; Cressey TN; Deflube LR; Ruedas JB; Connor JH; Ebihara H; Muhlberger E Antiviral Res 2017[Oct]; 146 (ä): 21-27 PMID28807685show ga Ebola virus (EBOV) causes a severe disease in humans with the potential for significant international public health consequences. Currently, treatments are limited to experimental vaccines and therapeutics. Therefore, research into prophylaxis and antiviral strategies to combat EBOV infections is of utmost importance. The requirement for high containment laboratories to study EBOV infection is a limiting factor for conducting EBOV research. To overcome this issue, minigenome systems have been used as valuable tools to study EBOV replication and transcription mechanisms and to screen for antiviral compounds at biosafety level 2. The most commonly used EBOV minigenome system relies on the ectopic expression of the T7 RNA polymerase (T7), which can be limiting for certain cell types. We have established an improved EBOV minigenome system that utilizes endogenous RNA polymerase II (pol II) as a driver for the synthesis of minigenome RNA. We show here that this system is as efficient as the T7-based minigenome system, but works in a wider range of cell types, including biologically relevant cell types such as bat cells. Importantly, we were also able to adapt this system to a reliable and cost-effective 96-well format antiviral screening assay with a Z-factor of 0.74, indicative of a robust assay. Using this format, we identified JG40, an inhibitor of Hsp70, as an inhibitor of EBOV replication, highlighting the potential for this system as a tool for antiviral drug screening. In summary, this updated EBOV minigenome system provides a convenient and effective means of advancing the field of EBOV research. |*Genome, Viral[MESH] |Animals[MESH] |Antiviral Agents/isolation & purification/*pharmacology[MESH] |DNA-Directed RNA Polymerases/genetics/metabolism[MESH] |Ebolavirus/*drug effects/enzymology/*genetics[MESH] |HSP72 Heat-Shock Proteins/antagonists & inhibitors[MESH] |Hemorrhagic Fever, Ebola/virology[MESH] |High-Throughput Screening Assays/economics/instrumentation/methods[MESH] |Humans[MESH] |Microbial Sensitivity Tests/economics/instrumentation/*methods[MESH] |RNA Polymerase II/*genetics/metabolism[MESH] |RNA, Viral/genetics[MESH] |Transcription, Genetic/drug effects[MESH] |Viral Proteins/genetics/metabolism[MESH]An RNA polymerase II-driven Ebola virus minigenome system as an advanc(epmc).pdf DeepDyve Pubget Overpricing