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10.1111/1348-0421.12505 http://scihub22266oqcxt.onion/10.1111/1348-0421.12505 28776750!ä!28776750 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Microbiol+Immunol 2017 ; 61 (9): 380-386 Nephropedia Template TP {{tp|p=28776750|t=2017. Generation of a non-transmissive Borna disease virus vector lacking both matrix and glycoprotein genes |pdf=|usr=}}{{28776750}} gab.com Text Generation of a non-transmissive Borna disease virus vector lacking both matrix and glycoprotein genes JO: Microbiol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=28776750 #FOAvip Borna disease virus (BoDV), a prototype of mammalian bornavirus, is a non-segmented, negative strand RNA virus that often causes severe neurological disorders in infected animals, including horses and sheep. Unique among animal RNA viruses, BoDV transcribes and replicates non-cytopathically in the cell nucleus, leading to establishment of long-lasting persistent infection. This striking feature of BoDV indicates its potential as an RNA virus vector system. It has previously been demonstrated by our team that recombinant BoDV (rBoDV) lacking an envelope glycoprotein (G) gene develops persistent infections in transduced cells without loss of the viral genome. In this study, a novel non-transmissive rBoDV, rBoDV DeltaMG, which lacks both matrix (M) and G genes in the genome, is reported. rBoDV-DeltaMG expressing green fluorescence protein (GFP), rBoDV DeltaMG-GFP, was efficiently generated in Vero/MG cells stably expressing both BoDV M and G proteins. Infection with rBoDV DeltaMG-GFP was persistently maintained in the parent Vero cells without propagation within cell culture. The optimal ratio of M and G for efficient viral particle production by transient transfection of M and G expression plasmids into cells persistently infected with rBoDV DeltaMG-GFP was also demonstrated. These findings indicate that the rBoDV DeltaMG-based BoDV vector may provide an extremely safe virus vector system and could be a novel strategy for investigating the function of M and G proteins and the host range of bornaviruses. Twit Text FOAVip Generation of a non-transmissive Borna disease virus vector lacking both matrix and glycoprotein genes ????Microbiol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=28776750 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28776750 #FOAvip English Wikipedia <ref>{{Cite pmid|28776750}}</ref> Generation of a non-transmissive Borna disease virus vector lacking both matrix and glycoprotein genes #MMPMID28776750 Fujino K; Yamamoto Y; Daito T; Makino A; Honda T; Tomonaga K Microbiol Immunol 2017[Sep]; 61 (9): 380-386 PMID28776750show ga Borna disease virus (BoDV), a prototype of mammalian bornavirus, is a non-segmented, negative strand RNA virus that often causes severe neurological disorders in infected animals, including horses and sheep. Unique among animal RNA viruses, BoDV transcribes and replicates non-cytopathically in the cell nucleus, leading to establishment of long-lasting persistent infection. This striking feature of BoDV indicates its potential as an RNA virus vector system. It has previously been demonstrated by our team that recombinant BoDV (rBoDV) lacking an envelope glycoprotein (G) gene develops persistent infections in transduced cells without loss of the viral genome. In this study, a novel non-transmissive rBoDV, rBoDV DeltaMG, which lacks both matrix (M) and G genes in the genome, is reported. rBoDV-DeltaMG expressing green fluorescence protein (GFP), rBoDV DeltaMG-GFP, was efficiently generated in Vero/MG cells stably expressing both BoDV M and G proteins. Infection with rBoDV DeltaMG-GFP was persistently maintained in the parent Vero cells without propagation within cell culture. The optimal ratio of M and G for efficient viral particle production by transient transfection of M and G expression plasmids into cells persistently infected with rBoDV DeltaMG-GFP was also demonstrated. These findings indicate that the rBoDV DeltaMG-based BoDV vector may provide an extremely safe virus vector system and could be a novel strategy for investigating the function of M and G proteins and the host range of bornaviruses. |Animals[MESH] |Borna Disease/*transmission/virology[MESH] |Borna disease virus/*genetics/pathogenicity[MESH] |Cell Line[MESH] |Chlorocebus aethiops[MESH] |Genetic Vectors/*genetics[MESH] |Genome, Viral/genetics[MESH] |Glycoproteins/genetics[MESH] |Green Fluorescent Proteins/*genetics[MESH] |HEK293 Cells[MESH] |Humans[MESH] |RNA, Viral/genetics[MESH] |Vero Cells[MESH] |Viral Envelope Proteins/*genetics[MESH] |Viral Matrix Proteins/*genetics[MESH]Generation of a non-transmissive Borna disease virus vector lacking bo(epmc).pdf DeepDyve Pubget Overpricing