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10.1016/j.vetmic.2017.06.012 http://scihub22266oqcxt.onion/10.1016/j.vetmic.2017.06.012 28757015!ä!28757015 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Vet+Microbiol 2017 ; 207 (ä): 143-148 Nephropedia Template TP {{tp|p=28757015|t=2017. Dual infections of CD163 expressing NPTr epithelial cells with influenza A virus and PRRSV |pdf=|usr=}}{{28757015}} gab.com Text Dual infections of CD163 expressing NPTr epithelial cells with influenza A virus and PRRSV JO: Vet Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=28757015 #FOAvip In the pig, respiratory co-infections involving various pathogens are far more frequent than single infections. Amongst respiratory viruses, swine influenza type A virus (swIAV) and porcine reproductive and respiratory syndrome virus (PRRSV) are frequently associated. Previously, we performed co-infections with swIAV and PRRSV in porcine alveolar macrophages (PAM) and precision cut lung slices (PCLS). With these two approaches it was practically impossible to have co-infections of the same cells as the main target cell of swIAV is the epithelial cell while the main target of PRRSV is the PAM. This constraint makes the study of interference between the two viruses difficult at the cellular level. In the current report, an epithelial cell line expressing, CD163, the main receptor of PRRSV was generated. This cell line receptive for both viruses was used to assess the interference between the two viruses. Results showed that swIAV as well as PRRSV, even if they interacted differently with the modified epithelial cells, were clearly interfering with each other regarding their replication when they infected a same cell with consequences within the cellular antiviral response. Our modified cell line, receptive to both viruses, can be used as a tool to assess interference between swIAV and PRRSV in a same cell as it probably happens in the porcine host. Twit Text FOAVip Dual infections of CD163 expressing NPTr epithelial cells with influenza A virus and PRRSV ????Vet Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=28757015 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28757015 #FOAvip English Wikipedia <ref>{{Cite pmid|28757015}}</ref> Dual infections of CD163 expressing NPTr epithelial cells with influenza A virus and PRRSV #MMPMID28757015 Provost C; Hamonic G; Gagnon CA; Meurens F Vet Microbiol 2017[Aug]; 207 (ä): 143-148 PMID28757015show ga In the pig, respiratory co-infections involving various pathogens are far more frequent than single infections. Amongst respiratory viruses, swine influenza type A virus (swIAV) and porcine reproductive and respiratory syndrome virus (PRRSV) are frequently associated. Previously, we performed co-infections with swIAV and PRRSV in porcine alveolar macrophages (PAM) and precision cut lung slices (PCLS). With these two approaches it was practically impossible to have co-infections of the same cells as the main target cell of swIAV is the epithelial cell while the main target of PRRSV is the PAM. This constraint makes the study of interference between the two viruses difficult at the cellular level. In the current report, an epithelial cell line expressing, CD163, the main receptor of PRRSV was generated. This cell line receptive for both viruses was used to assess the interference between the two viruses. Results showed that swIAV as well as PRRSV, even if they interacted differently with the modified epithelial cells, were clearly interfering with each other regarding their replication when they infected a same cell with consequences within the cellular antiviral response. Our modified cell line, receptive to both viruses, can be used as a tool to assess interference between swIAV and PRRSV in a same cell as it probably happens in the porcine host. |*Antigens, CD[MESH] |*Antigens, Differentiation, Myelomonocytic[MESH] |*Receptors, Cell Surface[MESH] |Animals[MESH] |Cell Line[MESH] |Coinfection[MESH] |Dogs[MESH] |Epithelial Cells/*metabolism/virology[MESH] |Gene Expression Regulation/immunology[MESH] |Influenza A virus/*physiology[MESH] |Porcine respiratory and reproductive syndrome virus/*physiology[MESH] |Swine[MESH] |Trachea/cytology[MESH] |Virus Cultivation[MESH]Dual infections of CD163 expressing NPTr epithelial cells with influen(epmc).pdf DeepDyve Pubget Overpricing