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10.1111/1348-0421.12498 http://scihub22266oqcxt.onion/10.1111/1348-0421.12498 28675506!7168434!28675506     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28675506&cmd=llinks): Failed to open stream: HTTP request failed! 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Determination of the cell tropism of serotype 1 feline infectious peritonitis virus using the spike affinity histochemistry in paraffin-embedded tissues |pdf=|usr=}}{{28675506}} gab.com Text Determination of the cell tropism of serotype 1 feline infectious peritonitis virus using the spike affinity histochemistry in paraffin-embedded tissues JO: Microbiol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=28675506 #FOAvip Unlike for serotype II feline coronaviruses (FCoV II), the cellular receptor for serotype I FCoV (FCoV I), the most prevalent FCoV serotype, is unknown. To provide a platform for assessing the pattern by which FCoV I attaches to its host receptor(s), HEK293 cell lines that stably express the ectodomains of the spike (S) proteins derived from a FCoV I feline enteric coronavirus strain UU7 (FECV UU7) and a feline infectious peritonitis virus strain UU4 (FIPV UU4) were established. Using the recombinant S proteins as probes to perform S protein affinity histochemistry in paraffin-embedded tissues, although no tissue or enteric binding of FECV UU7 S protein was detected, it was found that by immunohistochemistry that the tissue distribution of FIPV UU4 S protein-bound cells correlated with that of FIPV antigen-positive cells and lesions associated with FIP and that the affinity binding of FIPV UU4 S protein on macrophages was not affected by enzymatic removal of host cell-surface sialic acid with neuraminidase. These findings suggest that a factor(s) other than sialic acid contribute(s) to the macrophage tropism of FIPV strain UU4. This approach allowed obtaining more information about both virus-host cell interactions and the biological characteristics of the unidentified cellular receptor for FCoV I. Twit Text FOAVip Determination of the cell tropism of serotype 1 feline infectious peritonitis virus using the spike affinity histochemistry in paraffin-embedded tissues ????Microbiol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=28675506 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28675506 #FOAvip English Wikipedia <ref>{{Cite pmid|28675506}}</ref> Determination of the cell tropism of serotype 1 feline infectious peritonitis virus using the spike affinity histochemistry in paraffin-embedded tissues #MMPMID28675506 Cham TC; Chang YC; Tsai PS; Wu CH; Chen HW; Jeng CR; Pang VF; Chang HW Microbiol Immunol 2017[Aug]; 61 (8): 318-327 PMID28675506show ga Unlike for serotype II feline coronaviruses (FCoV II), the cellular receptor for serotype I FCoV (FCoV I), the most prevalent FCoV serotype, is unknown. To provide a platform for assessing the pattern by which FCoV I attaches to its host receptor(s), HEK293 cell lines that stably express the ectodomains of the spike (S) proteins derived from a FCoV I feline enteric coronavirus strain UU7 (FECV UU7) and a feline infectious peritonitis virus strain UU4 (FIPV UU4) were established. Using the recombinant S proteins as probes to perform S protein affinity histochemistry in paraffin-embedded tissues, although no tissue or enteric binding of FECV UU7 S protein was detected, it was found that by immunohistochemistry that the tissue distribution of FIPV UU4 S protein-bound cells correlated with that of FIPV antigen-positive cells and lesions associated with FIP and that the affinity binding of FIPV UU4 S protein on macrophages was not affected by enzymatic removal of host cell-surface sialic acid with neuraminidase. These findings suggest that a factor(s) other than sialic acid contribute(s) to the macrophage tropism of FIPV strain UU4. This approach allowed obtaining more information about both virus-host cell interactions and the biological characteristics of the unidentified cellular receptor for FCoV I. |*Virus Attachment[MESH] |Animals[MESH] |Cats[MESH] |Cell Line[MESH] |Coronavirus, Feline/*metabolism[MESH] |HEK293 Cells[MESH] |Host-Pathogen Interactions/physiology[MESH] |Humans[MESH] |Macrophages/virology[MESH] |N-Acetylneuraminic Acid/chemistry[MESH] |Protein Binding/genetics/physiology[MESH] |Receptors, Virus/*metabolism[MESH] |Serogroup[MESH] |Spike Glycoprotein, Coronavirus/genetics/*metabolism[MESH]Determination of the cell tropism of serotype 1 feline infectious peri(epmc).pdf DeepDyve Pubget Overpricing