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10.1021/acsnano.7b02267 http://scihub22266oqcxt.onion/10.1021/acsnano.7b02267 28605582!ä!28605582 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       ACS+Nano 2017 ; 11 (7): 6959-6967 Nephropedia Template TP {{tp|p=28605582|t=2017. Assessing Antibiotic Permeability of Gram-Negative Bacteria via Nanofluidics |pdf=|usr=}}{{28605582}} gab.com Text Assessing Antibiotic Permeability of Gram-Negative Bacteria via Nanofluidics JO: ACS Nano http://www.kidney.de/pget.php?&tw=1&pmid=28605582 #FOAvip While antibiotic resistance is increasing rapidly, drug discovery has proven to be extremely difficult. Antibiotic resistance transforms some bacterial infections into deadly medical conditions. A significant challenge in antibiotic discovery is designing potent molecules that enter Gram-negative bacteria and also avoid active efflux mechanisms. Critical analysis in rational drug design has been hindered by the lack of effective analytical tools to analyze the bacterial membrane permeability of small molecules. We design, fabricate, and characterize the nanofluidic device that actively loads more than 200 single bacterial cells in a nanochannel array. We demonstrate a gigaohm seal between the nanochannel walls and the loaded bacteria, restricting small molecule transport to only occur through the bacterial cell envelope. Quantitation of clindamycin translocation through wild-type and efflux-deficient (DeltatolC) Escherichia coli strains via nanofluidic-interfaced liquid chromatography mass spectrometry shows higher levels of translocation for wild-type E. coli than for an efflux-deficient strain. We believe that the assessment of compound permeability in Gram-negative bacteria via the nanofluidic analysis platform will be an impactful tool for compound permeation and efflux studies in bacteria to assist rational antibiotic design. Twit Text FOAVip Assessing Antibiotic Permeability of Gram-Negative Bacteria via Nanofluidics ????ACS Nano http://www.kidney.de/pget.php?&tw=1&pmid=28605582 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28605582 #FOAvip English Wikipedia <ref>{{Cite pmid|28605582}}</ref> Assessing Antibiotic Permeability of Gram-Negative Bacteria via Nanofluidics #MMPMID28605582 Hong S; Moritz TJ; Rath CM; Tamrakar P; Lee P; Krucker T; Lee LP ACS Nano 2017[Jul]; 11 (7): 6959-6967 PMID28605582show ga While antibiotic resistance is increasing rapidly, drug discovery has proven to be extremely difficult. Antibiotic resistance transforms some bacterial infections into deadly medical conditions. A significant challenge in antibiotic discovery is designing potent molecules that enter Gram-negative bacteria and also avoid active efflux mechanisms. Critical analysis in rational drug design has been hindered by the lack of effective analytical tools to analyze the bacterial membrane permeability of small molecules. We design, fabricate, and characterize the nanofluidic device that actively loads more than 200 single bacterial cells in a nanochannel array. We demonstrate a gigaohm seal between the nanochannel walls and the loaded bacteria, restricting small molecule transport to only occur through the bacterial cell envelope. Quantitation of clindamycin translocation through wild-type and efflux-deficient (DeltatolC) Escherichia coli strains via nanofluidic-interfaced liquid chromatography mass spectrometry shows higher levels of translocation for wild-type E. coli than for an efflux-deficient strain. We believe that the assessment of compound permeability in Gram-negative bacteria via the nanofluidic analysis platform will be an impactful tool for compound permeation and efflux studies in bacteria to assist rational antibiotic design. |*Lab-On-A-Chip Devices[MESH] |Anti-Bacterial Agents/*metabolism/pharmacokinetics[MESH] |Clindamycin/*metabolism/pharmacokinetics[MESH] |Drug Discovery/instrumentation[MESH] |Drug Resistance, Multiple, Bacterial[MESH] |Equipment Design[MESH] |Escherichia coli Infections/*drug therapy/microbiology[MESH] |Escherichia coli/drug effects/*metabolism[MESH] |Gram-Negative Bacteria/metabolism[MESH] |Humans[MESH] |Nanotechnology/*instrumentation[MESH]Assessing Antibiotic Permeability of Gram-Negative Bacteria via Nanofl(epmc).pdf DeepDyve Pubget Overpricing