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10.1186/s40478-017-0447-3 http://scihub22266oqcxt.onion/10.1186/s40478-017-0447-3 28554330/?report=reader!5448146!28554330     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Acta+Neuropathol+Commun 2017 ; 5 (1): 40 Nephropedia Template TP {{tp|p=28554330|t=2017. Rho-associated protein kinase 2 (ROCK2): a new target of autoimmunity in paraneoplastic encephalitis |pdf=|usr=}}{{28554330}} gab.com Text Rho-associated protein kinase 2 (ROCK2): a new target of autoimmunity in paraneoplastic encephalitis JO: Acta Neuropathol Commun http://www.kidney.de/pget.php?&tw=1&pmid=28554330 #FOAvip Onconeural antibodies are associated with cancer and paraneoplastic encephalitis. While their pathogenic role is still largely unknown, their high diagnostic value is undisputed. In this study we describe the discovery of a novel target of autoimmunity in an index case of paraneoplastic encephalitis associated with urogenital cancer.A 75-year-old man with a history of invasive bladder carcinoma 6 years ago with multiple recurrences and a newly discovered renal cell carcinoma presented with seizures and progressive cognitive decline followed by super-refractory status epilepticus. Clinical and ancillary findings including brain biopsy suggested paraneoplastic encephalitis. Immunohistochemistry of the brain biopsy was used to characterize the inflammatory response. Indirect immunofluorescence assay (IFA) was used for autoantibody screening. The autoantigen was identified by histo-immunoprecipitation and mass spectrometry and was validated by expressing the recombinant antigen in HEK293 cells and neutralization tests. Sera from 125 control patients were screened using IFA to test for the novel autoantibodies.IFA analysis of serum revealed a novel autoantibody against brain tissue. An intracellular enzyme, Rho-associated protein kinase 2 (ROCK2), was identified as target-antigen. ROCK2 was expressed in affected brain tissue and archival bladder tumor samples of this patient. Brain histopathology revealed appositions of cytotoxic CD8(+) T cells on ROCK2-positive neurons. ROCK2 antibodies were not found in the sera of 20 patients with bladder cancer and 17 with renal cancer, both without neurological symptoms, 49 healthy controls, and 39 patients with other antineuronal autoantibodies. In conclusion, novel onconeural antibodies targeting ROCK2 are associated with paraneoplastic encephalitis and should be screened for when paraneoplastic neurological syndromes, especially in patients with urogenital cancers, occur. Twit Text FOAVip Rho-associated protein kinase 2 (ROCK2): a new target of autoimmunity in paraneoplastic encephalitis ????Acta Neuropathol Commun http://www.kidney.de/pget.php?&tw=1&pmid=28554330 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28554330 #FOAvip English Wikipedia <ref>{{Cite pmid|28554330}}</ref> Rho-associated protein kinase 2 (ROCK2): a new target of autoimmunity in paraneoplastic encephalitis #MMPMID28554330 Popkirov S; Ayzenberg I; Hahn S; Bauer J; Denno Y; Rieckhoff N; Radzimski C; Hans VH; Berg S; Roghmann F; Noldus J; Bien CG; Skodda S; Wellmer J; Stocker W; Krogias C; Gold R; Schlegel U; Probst C; Komorowski L; Miske R; Kleiter I Acta Neuropathol Commun 2017[May]; 5 (1): 40 PMID28554330show ga Onconeural antibodies are associated with cancer and paraneoplastic encephalitis. While their pathogenic role is still largely unknown, their high diagnostic value is undisputed. In this study we describe the discovery of a novel target of autoimmunity in an index case of paraneoplastic encephalitis associated with urogenital cancer.A 75-year-old man with a history of invasive bladder carcinoma 6 years ago with multiple recurrences and a newly discovered renal cell carcinoma presented with seizures and progressive cognitive decline followed by super-refractory status epilepticus. Clinical and ancillary findings including brain biopsy suggested paraneoplastic encephalitis. Immunohistochemistry of the brain biopsy was used to characterize the inflammatory response. Indirect immunofluorescence assay (IFA) was used for autoantibody screening. The autoantigen was identified by histo-immunoprecipitation and mass spectrometry and was validated by expressing the recombinant antigen in HEK293 cells and neutralization tests. Sera from 125 control patients were screened using IFA to test for the novel autoantibodies.IFA analysis of serum revealed a novel autoantibody against brain tissue. An intracellular enzyme, Rho-associated protein kinase 2 (ROCK2), was identified as target-antigen. ROCK2 was expressed in affected brain tissue and archival bladder tumor samples of this patient. Brain histopathology revealed appositions of cytotoxic CD8(+) T cells on ROCK2-positive neurons. ROCK2 antibodies were not found in the sera of 20 patients with bladder cancer and 17 with renal cancer, both without neurological symptoms, 49 healthy controls, and 39 patients with other antineuronal autoantibodies. In conclusion, novel onconeural antibodies targeting ROCK2 are associated with paraneoplastic encephalitis and should be screened for when paraneoplastic neurological syndromes, especially in patients with urogenital cancers, occur. |Aged[MESH] |Autoantibodies/blood/*immunology[MESH] |Autoimmune Diseases of the Nervous System/*enzymology/immunology[MESH] |Autoimmunity[MESH] |Brain/enzymology/immunology[MESH] |Carcinoma/immunology[MESH] |Encephalitis/*enzymology/*immunology[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Kidney Neoplasms/immunology[MESH] |Male[MESH] |Paraneoplastic Syndromes, Nervous System/*enzymology/*immunology[MESH] |Urinary Bladder Neoplasms/immunology[MESH]Rho-associated protein kinase 2 (ROCK2): a new target of autoimmunity (epmc).pdf DeepDyve Pubget Overpricing