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10.1002/jcla.22230

http://scihub22266oqcxt.onion/10.1002/jcla.22230
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suck abstract from ncbi


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pmid28397965      J+Clin+Lab+Anal 2018 ; 32 (2): ä
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  • Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real-Q RV #MMPMID28397965
  • Yun SG; Kim MY; Choi JM; Lee CK; Lim CS; Cho Y; Suh IB
  • J Clin Lab Anal 2018[Feb]; 32 (2): ä PMID28397965show ga
  • BACKGROUND: Due to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT-PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT-PCR assays for the detection of respiratory viruses. METHODS: A total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real-Q RV (RQ). The additional tests for the discrepant results were conducted by repeat RV assay or monoplex PCR coupled direct sequencing. Data analysis using percent agreement, kappa, and prevalence-adjusted and bias-adjusted kappa (PABAK) values was performed for comparisons among the three RV assays. RESULTS: Of the 201 samples, AP, AD, and RQ detected 105 (52.2%), 99 (49.3%), and 95 (47.3%) positive cases respectively. The overall agreement, kappa, and PABAK values for the three assays ranged between 97%-98%, 0.76-0.86, and 0.93-0.96 respectively. The performance of the three assays was very similar, with 94%-100% agreement for all comparisons, each virus types. The additional testing of samples showed discrepant results demonstrating that AD assay had the highest rate of concordance with original results. CONCLUSIONS: We suggest that all multiplex assay would be suitable for the detection of for respiratory viruses in clinical setting.
  • |Adolescent[MESH]
  • |Adult[MESH]
  • |Humans[MESH]
  • |Male[MESH]
  • |Molecular Diagnostic Techniques/*methods[MESH]
  • |Multiplex Polymerase Chain Reaction/*methods[MESH]
  • |Reproducibility of Results[MESH]
  • |Respiratory Tract Infections/diagnosis/*virology[MESH]
  • |Sensitivity and Specificity[MESH]
  • |Virus Diseases/diagnosis/*virology[MESH]
  • |Viruses/*genetics[MESH]


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