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10.1002/jmv.24780 http://scihub22266oqcxt.onion/10.1002/jmv.24780 28191658!7166611!28191658     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28191658&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2017 ; 89 (8): 1330-1338 Nephropedia Template TP {{tp|p=28191658|t=2017. Interpatient mutational spectrum of human coronavirus-OC43 revealed by illumina sequencing |pdf=|usr=}}{{28191658}} gab.com Text Interpatient mutational spectrum of human coronavirus-OC43 revealed by illumina sequencing JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=28191658 #FOAvip Human coronaviruses (HCoV) are RNA viruses that cause respiratory tract infections with viral replication of limited duration. The host and viral population heterogeneity could influence clinical phenotypes. Employing long RT-PCR with Illumina sequencing, we quantified the gene mutation load at 0.5% mutation frequency for the 4529 bp-domain spanning the Spike gene (4086 bp) of HCoV-OC43 in four upper respiratory clinical specimens obtained during acute illness. There were a total of 121 mutations for all four HCoV samples with the average number of mutations at 30.3 +/- 10.2, which is significantly higher than that expected from the Illumina sequencing error rate. There were two mutation peaks, one at the 5' end and the other near position 1 550 in the S1 subunit. Two coronavirus samples were genotype B and two were genotype D, clustering with HCoV-OC43 strain AY391777 in neighbor-joining tree phylogenetic analysis. Nonsynonymous mutations were 76.1 +/- 14% of mutation load. Although lower than other RNA viruses such as hepatitis C virus, HCoV-OC43 did exhibit quasi-species. The rate of nonsynonymous mutations was higher in the HCoV-OC43 isolates than in hepatitis C (HCV) virus genotype 1a isolates analyzed for comparison in this study. These characteristics of HCoV-OC43 may affect viral replication dynamics, receptor binding, antigenicity, evolution, transmission, and clinical illness. Twit Text FOAVip Interpatient mutational spectrum of human coronavirus-OC43 revealed by illumina sequencing ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=28191658 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28191658 #FOAvip English Wikipedia <ref>{{Cite pmid|28191658}}</ref> Interpatient mutational spectrum of human coronavirus-OC43 revealed by illumina sequencing #MMPMID28191658 Gorse GJ; Patel GB; Fan X J Med Virol 2017[Aug]; 89 (8): 1330-1338 PMID28191658show ga Human coronaviruses (HCoV) are RNA viruses that cause respiratory tract infections with viral replication of limited duration. The host and viral population heterogeneity could influence clinical phenotypes. Employing long RT-PCR with Illumina sequencing, we quantified the gene mutation load at 0.5% mutation frequency for the 4529 bp-domain spanning the Spike gene (4086 bp) of HCoV-OC43 in four upper respiratory clinical specimens obtained during acute illness. There were a total of 121 mutations for all four HCoV samples with the average number of mutations at 30.3 +/- 10.2, which is significantly higher than that expected from the Illumina sequencing error rate. There were two mutation peaks, one at the 5' end and the other near position 1 550 in the S1 subunit. Two coronavirus samples were genotype B and two were genotype D, clustering with HCoV-OC43 strain AY391777 in neighbor-joining tree phylogenetic analysis. Nonsynonymous mutations were 76.1 +/- 14% of mutation load. Although lower than other RNA viruses such as hepatitis C virus, HCoV-OC43 did exhibit quasi-species. The rate of nonsynonymous mutations was higher in the HCoV-OC43 isolates than in hepatitis C (HCV) virus genotype 1a isolates analyzed for comparison in this study. These characteristics of HCoV-OC43 may affect viral replication dynamics, receptor binding, antigenicity, evolution, transmission, and clinical illness. |*Genetic Variation[MESH] |*Mutation[MESH] |Adult[MESH] |Aged, 80 and over[MESH] |Cluster Analysis[MESH] |Coronavirus Infections/*virology[MESH] |Coronavirus OC43, Human/*classification/*genetics/isolation & purification[MESH] |Genotype[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Mutation Rate[MESH] |Phylogeny[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |Sequence Analysis, DNA[MESH]Interpatient mutational spectrum of human coronavirus-OC43 revealed by(epmc).pdf DeepDyve Pubget Overpricing