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10.1038/srep42230 http://scihub22266oqcxt.onion/10.1038/srep42230 28186116!5301479!28186116     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2017 ; 7 (ä): 42230 Nephropedia Template TP {{tp|p=28186116|t=2017. Targeting of phage particles towards endothelial cells by antibodies selected through a multi-parameter selection strategy |pdf=|usr=}}{{28186116}} gab.com Text Targeting of phage particles towards endothelial cells by antibodies selected through a multi-parameter selection strategy JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28186116 #FOAvip One of the hallmarks of cancer is sustained angiogenesis. Here, normal endothelial cells are activated, and their formation of new blood vessels leads to continued tumour growth. An improved patient condition is often observed when angiogenesis is prevented or normalized through targeting of these genomically stable endothelial cells. However, intracellular targets constitute a challenge in therapy, as the agents modulating these targets have to be delivered and internalized specifically to the endothelial cells. Selection of antibodies binding specifically to certain cell types is well established. It is nonetheless a challenge to ensure that the binding of antibodies to the target cell will mediate internalization. Previously selection of such antibodies has been performed targeting cancer cell lines; most often using either monovalent display or polyvalent display. In this article, we describe selections that isolate internalizing antibodies by sequential combining monovalent and polyvalent display using two types of helper phages, one which increases display valence and one which reduces background. One of the selected antibodies was found to mediate internalization into human endothelial cells, although our results confirms that the single stranded nature of the DNA packaged into phage particles may limit applications aimed at targeting nucleic acids in mammalian cells. Twit Text FOAVip Targeting of phage particles towards endothelial cells by antibodies selected through a multi-parameter selection strategy ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28186116 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28186116 #FOAvip English Wikipedia <ref>{{Cite pmid|28186116}}</ref> Targeting of phage particles towards endothelial cells by antibodies selected through a multi-parameter selection strategy #MMPMID28186116 Mandrup OA; Lykkemark S; Kristensen P Sci Rep 2017[Feb]; 7 (ä): 42230 PMID28186116show ga One of the hallmarks of cancer is sustained angiogenesis. Here, normal endothelial cells are activated, and their formation of new blood vessels leads to continued tumour growth. An improved patient condition is often observed when angiogenesis is prevented or normalized through targeting of these genomically stable endothelial cells. However, intracellular targets constitute a challenge in therapy, as the agents modulating these targets have to be delivered and internalized specifically to the endothelial cells. Selection of antibodies binding specifically to certain cell types is well established. It is nonetheless a challenge to ensure that the binding of antibodies to the target cell will mediate internalization. Previously selection of such antibodies has been performed targeting cancer cell lines; most often using either monovalent display or polyvalent display. In this article, we describe selections that isolate internalizing antibodies by sequential combining monovalent and polyvalent display using two types of helper phages, one which increases display valence and one which reduces background. One of the selected antibodies was found to mediate internalization into human endothelial cells, although our results confirms that the single stranded nature of the DNA packaged into phage particles may limit applications aimed at targeting nucleic acids in mammalian cells. |Antibodies/*pharmacology[MESH] |Bacteriophages/*metabolism[MESH] |Cell Line[MESH] |DNA/metabolism[MESH] |Endocytosis[MESH] |Endothelial Cells/drug effects/*metabolism[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Fibroblasts/cytology[MESH] |Gene Transfer Techniques[MESH] |Humans[MESH] |Mesenchymal Stem Cells/cytology[MESH] |Microvessels/cytology[MESH] |Peptide Library[MESH]Targeting of phage particles towards endothelial cells by antibodies s(epmc).pdf DeepDyve Pubget Overpricing