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10.1002/jmv.24772 http://scihub22266oqcxt.onion/10.1002/jmv.24772 28165146!7167024!28165146     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2017 ; 89 (7): 1201-1207 Nephropedia Template TP {{tp|p=28165146|t=2017. Enterovirus D68 detection in respiratory specimens: Association with severe disease |pdf=|usr=}}{{28165146}} gab.com Text Enterovirus D68 detection in respiratory specimens: Association with severe disease JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=28165146 #FOAvip Molecular techniques increased the number of documented respiratory infections. In a substantial number of cases the causative agent remains undetected. Since August 2014, an increase in Enterovirus(EV)-D68 infections was reported. We aimed to investigate epidemiology and clinical relevance of EV-D68. From June to December 2014 and from September to December 2015, 803 and 847 respiratory specimens, respectively, were tested for respiratory viruses with a multiplex RT-PCR. This multiplex RT-PCR does not detect EV-D68. Therefore, 457 (2014) and 343 (2015) specimens with negative results were submitted to an EV-specific-RT-PCR. EV-positive specimens were tested with an EV-D68-specific-RT-PCR and genotyped. Eleven specimens of 2014 tested positive in the EV-specific-RT-PCR and of these seven were positive in the EV-D68-specific-RT-PCR. Typing confirmed these as EV-D68. Median age of EV-D68-positive patients was 3 years (1 month-91 years). Common symptoms included fever (n = 6, 86%), respiratory distress (n = 5, 71%), and cough (n = 4, 57%). All EV-D68-positive patients were admitted to hospital, 4 (57%) were admitted to intensive care units and 6 (86%) received oxygen. One patient suffered from acute flaccid paralysis. Seven specimens of 2015 were positive in the EV-specific-RT-PCR but negative in the EV-D68-specific-RT-PCR. In conclusion, use of an EV-specific-RT-PCR allowed us to detect EV-D68 circulation in autumn 2014 that was not detected by the multiplex RT-PCR and was associated with severe disease. Twit Text FOAVip Enterovirus D68 detection in respiratory specimens: Association with severe disease ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=28165146 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28165146 #FOAvip English Wikipedia <ref>{{Cite pmid|28165146}}</ref> Enterovirus D68 detection in respiratory specimens: Association with severe disease #MMPMID28165146 Engelmann I; Fatoux M; Lazrek M; Alidjinou EK; Mirand A; Henquell C; Dewilde A; Hober D J Med Virol 2017[Jul]; 89 (7): 1201-1207 PMID28165146show ga Molecular techniques increased the number of documented respiratory infections. In a substantial number of cases the causative agent remains undetected. Since August 2014, an increase in Enterovirus(EV)-D68 infections was reported. We aimed to investigate epidemiology and clinical relevance of EV-D68. From June to December 2014 and from September to December 2015, 803 and 847 respiratory specimens, respectively, were tested for respiratory viruses with a multiplex RT-PCR. This multiplex RT-PCR does not detect EV-D68. Therefore, 457 (2014) and 343 (2015) specimens with negative results were submitted to an EV-specific-RT-PCR. EV-positive specimens were tested with an EV-D68-specific-RT-PCR and genotyped. Eleven specimens of 2014 tested positive in the EV-specific-RT-PCR and of these seven were positive in the EV-D68-specific-RT-PCR. Typing confirmed these as EV-D68. Median age of EV-D68-positive patients was 3 years (1 month-91 years). Common symptoms included fever (n = 6, 86%), respiratory distress (n = 5, 71%), and cough (n = 4, 57%). All EV-D68-positive patients were admitted to hospital, 4 (57%) were admitted to intensive care units and 6 (86%) received oxygen. One patient suffered from acute flaccid paralysis. Seven specimens of 2015 were positive in the EV-specific-RT-PCR but negative in the EV-D68-specific-RT-PCR. In conclusion, use of an EV-specific-RT-PCR allowed us to detect EV-D68 circulation in autumn 2014 that was not detected by the multiplex RT-PCR and was associated with severe disease. |Adolescent[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |Bronchoalveolar Lavage[MESH] |Child[MESH] |Child, Preschool[MESH] |Cough[MESH] |Disease Outbreaks[MESH] |Enterovirus D, Human/classification/*genetics/*isolation & purification[MESH] |Enterovirus Infections/epidemiology/physiopathology/*virology[MESH] |Female[MESH] |France/epidemiology[MESH] |Genotype[MESH] |Hospitalization[MESH] |Humans[MESH] |Infant[MESH] |Lung/virology[MESH] |Male[MESH] |Middle Aged[MESH] |Multiplex Polymerase Chain Reaction[MESH] |Paralysis/etiology/virology[MESH] |Real-Time Polymerase Chain Reaction[MESH] |Respiratory Tract Infections/epidemiology/physiopathology/*virology[MESH] |Severity of Illness Index[MESH]Enterovirus D68 detection in respiratory specimens: Association with s(epmc).pdf DeepDyve Pubget Overpricing