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10.3892/etm.2016.3963 http://scihub22266oqcxt.onion/10.3892/etm.2016.3963 28123510!5245135!28123510     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28123510&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Exp+Ther+Med 2017 ; 13 (1): 327-334 Nephropedia Template TP {{tp|p=28123510|t=2017. Effects of Prunus mume Siebold & Zucc in the pacemaking activity of interstitial cells of Cajal in murine small intestine |pdf=|usr=}}{{28123510}} gab.com Text Effects of Prunus mume Siebold & Zucc in the pacemaking activity of interstitial cells of Cajal in murine small intestine JO: Exp Ther Med http://www.kidney.de/pget.php?&tw=1&pmid=28123510 #FOAvip Interstitial cells of Cajal (ICCs) function as pacemaker cells in the gastrointestinal (GI) tract and therefore, serve an important role in regulating GI motility. The effects of a species of plum (Prunus mume Siebold & Zucc.) on cultured ICC cluster-induced pacemaker potentials in the mouse small intestine were investigated, and the effects of a methanolic extract of Prunus mume (m-PM) on ICC pacemaker activities were examined using the whole-cell patch-clamp technique. ICC pacemaker membrane potentials were depolarized by m-PM in a concentration dependent manner in current clamp mode. 4-Diphenylacetoxy-N-methyl-piperidine methiodide, which is a muscarinic 3 (M(3)) receptor antagonist, was able to block m-PM-induced pacemaker potential increases, whereas methoctramine, which is a muscarinic 2 (M(2)) receptor antagonist, was not. When 1 mM guanosine diphosphate beta-5 was present in the pipette solution, m-PM induced slight pacemaker depolarization. Following pretreatment in bath solution of Ca(2+)-free solution or a Ca(2+)-ATPase inhibitor in endoplasmic reticulum, the pacemaker currents were inhibited. Furthermore, pretreatment with PD98059, SB203580 or SP600125, which is a c-jun NH2-terminal kinase inhibitor, blocked m-PM-induced ICC potential depolarization. Furthermore, m-PM inhibited transient receptor potential melastatin (TRPM) 7 channels, but did not affect Ca(2+)-activated Cl(-) channels. These results suggest that m-PM is able to modulate pacemaker potentials through the muscarinic M(3) receptor, via G-protein and external and internal Ca(2+), in a mitogen-activated protein kinase and TRPM7-dependent manner. Therefore, m-PM may provide a basis for the development of a novel gastroprokinetic agent. Twit Text FOAVip Effects of Prunus mume Siebold & Zucc in the pacemaking activity of interstitial cells of Cajal in murine small intestine ????Exp Ther Med http://www.kidney.de/pget.php?&tw=1&pmid=28123510 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28123510 #FOAvip English Wikipedia <ref>{{Cite pmid|28123510}}</ref> Effects of Prunus mume Siebold & Zucc in the pacemaking activity of interstitial cells of Cajal in murine small intestine #MMPMID28123510 Lee SW; Kim SJ; Kim H; Yang D; Kim HJ; Kim BJ Exp Ther Med 2017[Jan]; 13 (1): 327-334 PMID28123510show ga Interstitial cells of Cajal (ICCs) function as pacemaker cells in the gastrointestinal (GI) tract and therefore, serve an important role in regulating GI motility. The effects of a species of plum (Prunus mume Siebold & Zucc.) on cultured ICC cluster-induced pacemaker potentials in the mouse small intestine were investigated, and the effects of a methanolic extract of Prunus mume (m-PM) on ICC pacemaker activities were examined using the whole-cell patch-clamp technique. ICC pacemaker membrane potentials were depolarized by m-PM in a concentration dependent manner in current clamp mode. 4-Diphenylacetoxy-N-methyl-piperidine methiodide, which is a muscarinic 3 (M(3)) receptor antagonist, was able to block m-PM-induced pacemaker potential increases, whereas methoctramine, which is a muscarinic 2 (M(2)) receptor antagonist, was not. When 1 mM guanosine diphosphate beta-5 was present in the pipette solution, m-PM induced slight pacemaker depolarization. Following pretreatment in bath solution of Ca(2+)-free solution or a Ca(2+)-ATPase inhibitor in endoplasmic reticulum, the pacemaker currents were inhibited. Furthermore, pretreatment with PD98059, SB203580 or SP600125, which is a c-jun NH2-terminal kinase inhibitor, blocked m-PM-induced ICC potential depolarization. Furthermore, m-PM inhibited transient receptor potential melastatin (TRPM) 7 channels, but did not affect Ca(2+)-activated Cl(-) channels. These results suggest that m-PM is able to modulate pacemaker potentials through the muscarinic M(3) receptor, via G-protein and external and internal Ca(2+), in a mitogen-activated protein kinase and TRPM7-dependent manner. Therefore, m-PM may provide a basis for the development of a novel gastroprokinetic agent. ?Effects of Prunus mume Siebold & Zucc in the pacemaking activity of i(epmc).pdf DeepDyve Pubget Overpricing