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10.1111/1556-4029.13415

http://scihub22266oqcxt.onion/10.1111/1556-4029.13415
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28120330!7166375!28120330
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suck abstract from ncbi


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pmid28120330      J+Forensic+Sci 2017 ; 62 (5): 1223-1228
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  • Use of an Automated Nested Multiplex Respiratory Pathogen PCR Panel Postmortem in the Pediatric Forensic Setting #MMPMID28120330
  • Baker T; Schandl C; Presnell SE; Madory J; Nolte FS; Batalis N
  • J Forensic Sci 2017[Sep]; 62 (5): 1223-1228 PMID28120330show ga
  • Respiratory pathogens have been detected in forensic investigations using multiple techniques; however, no study has examined the use of automated, nested, multiplex polymerase chain reaction (ANM-PCR), commonly used in living patients, in the forensic setting. This retrospective study assessed the utility of ANM-PCR in detecting respiratory pathogens in the pediatric forensic setting. Respiratory samples from 35 cases were tested for up to 20 respiratory pathogens. 51.4% of these cases yielded a positive ANM-PCR result, 20% of which were considered the cause of or contributory to death. The most commonly detected pathogens were rhinovirus/enterovirus and respiratory syncytial virus, and these were the only pathogens determined to play a significant role in cause of death. The sampled sites and postmortem intervals tested did not affect the likelihood of a positive or negative test. ANM-PCR panels are effective, affordable, and rapid ancillary tools in evaluating cause of death in the forensic pediatric population.
  • |Adenoviridae/genetics/isolation & purification[MESH]
  • |Child[MESH]
  • |Child, Preschool[MESH]
  • |Coronavirus/genetics/isolation & purification[MESH]
  • |DNA, Viral/*genetics[MESH]
  • |Enterovirus/genetics/isolation & purification[MESH]
  • |Female[MESH]
  • |Forensic Pathology[MESH]
  • |Humans[MESH]
  • |Infant[MESH]
  • |Infant, Newborn[MESH]
  • |Male[MESH]
  • |Multiplex Polymerase Chain Reaction/*methods[MESH]
  • |Respiratory Syncytial Virus, Human/genetics/isolation & purification[MESH]
  • |Respiratory Tract Infections/*virology[MESH]
  • |Retrospective Studies[MESH]


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