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10.1128/MCB.00508-16 http://scihub22266oqcxt.onion/10.1128/MCB.00508-16 28052936!5359427!28052936     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 298.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Cell+Biol 2017 ; 37 (7): ä Nephropedia Template TP {{tp|p=28052936|t=2017. KLHL3 Knockout Mice Reveal the Physiological Role of KLHL3 and the Pathophysiology of Pseudohypoaldosteronism Type II Caused by Mutant KLHL3 |pdf=|usr=}}{{28052936}} gab.com Text KLHL3 Knockout Mice Reveal the Physiological Role of KLHL3 and the Pathophysiology of Pseudohypoaldosteronism Type II Caused by Mutant KLHL3 JO: Mol Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=28052936 #FOAvip Mutations in the with-no-lysine kinase 1 (WNK1), WNK4, kelch-like 3 (KLHL3), and cullin3 (CUL3) genes are known to cause the hereditary disease pseudohypoaldosteronism type II (PHAII). It was recently demonstrated that this results from the defective degradation of WNK1 and WNK4 by the KLHL3/CUL3 ubiquitin ligase complex. However, the other physiological in vivo roles of KLHL3 remain unclear. Therefore, here we generated KLHL3(-/-) mice that expressed beta-galactosidase (beta-Gal) under the control of the endogenous KLHL3 promoter. Immunoblots of beta-Gal and LacZ staining revealed that KLHL3 was expressed in some organs, such as brain. However, the expression levels of WNK kinases were not increased in any of these organs other than the kidney, where WNK1 and WNK4 increased in KLHL3(-/-) mice but not in KLHL3(+/-) mice. KLHL3(-/-) mice also showed PHAII-like phenotypes, whereas KLHL3(+/-) mice did not. This clearly demonstrates that the heterozygous deletion of KLHL3 was not sufficient to cause PHAII, indicating that autosomal dominant type PHAII is caused by the dominant negative effect of mutant KLHL3. We further demonstrated that the dimerization of KLHL3 can explain this dominant negative effect. These findings could help us to further understand the physiological roles of KLHL3 and the pathophysiology of PHAII caused by mutant KLHL3. Twit Text FOAVip KLHL3 Knockout Mice Reveal the Physiological Role of KLHL3 and the Pathophysiology of Pseudohypoaldosteronism Type II Caused by Mutant KLHL3 ????Mol Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=28052936 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28052936 #FOAvip English Wikipedia <ref>{{Cite pmid|28052936}}</ref> KLHL3 Knockout Mice Reveal the Physiological Role of KLHL3 and the Pathophysiology of Pseudohypoaldosteronism Type II Caused by Mutant KLHL3 #MMPMID28052936 Sasaki E; Susa K; Mori T; Isobe K; Araki Y; Inoue Y; Yoshizaki Y; Ando F; Mori Y; Mandai S; Zeniya M; Takahashi D; Nomura N; Rai T; Uchida S; Sohara E Mol Cell Biol 2017[Apr]; 37 (7): ä PMID28052936show ga Mutations in the with-no-lysine kinase 1 (WNK1), WNK4, kelch-like 3 (KLHL3), and cullin3 (CUL3) genes are known to cause the hereditary disease pseudohypoaldosteronism type II (PHAII). It was recently demonstrated that this results from the defective degradation of WNK1 and WNK4 by the KLHL3/CUL3 ubiquitin ligase complex. However, the other physiological in vivo roles of KLHL3 remain unclear. Therefore, here we generated KLHL3(-/-) mice that expressed beta-galactosidase (beta-Gal) under the control of the endogenous KLHL3 promoter. Immunoblots of beta-Gal and LacZ staining revealed that KLHL3 was expressed in some organs, such as brain. However, the expression levels of WNK kinases were not increased in any of these organs other than the kidney, where WNK1 and WNK4 increased in KLHL3(-/-) mice but not in KLHL3(+/-) mice. KLHL3(-/-) mice also showed PHAII-like phenotypes, whereas KLHL3(+/-) mice did not. This clearly demonstrates that the heterozygous deletion of KLHL3 was not sufficient to cause PHAII, indicating that autosomal dominant type PHAII is caused by the dominant negative effect of mutant KLHL3. We further demonstrated that the dimerization of KLHL3 can explain this dominant negative effect. These findings could help us to further understand the physiological roles of KLHL3 and the pathophysiology of PHAII caused by mutant KLHL3. |Adaptor Proteins, Signal Transducing[MESH] |Animals[MESH] |Gene Knock-In Techniques[MESH] |Genes, Dominant[MESH] |Kidney/enzymology/pathology[MESH] |Mice, Inbred C57BL[MESH] |Mice, Knockout[MESH] |Microfilament Proteins/deficiency/*genetics[MESH] |Models, Biological[MESH] |Mutant Proteins/metabolism[MESH] |Mutation/*genetics[MESH] |Phenotype[MESH] |Phosphorylation[MESH] |Protein Multimerization[MESH] |Protein Serine-Threonine Kinases/metabolism[MESH] |Pseudohypoaldosteronism/blood/*genetics/*physiopathology[MESH]KLHL3 Knockout Mice Reveal the Physiological Role of KLHL3 and the Pa(epmc).pdf DeepDyve Pubget Overpricing