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10.1128/JVI.63.11.4814-4823.1989 http://scihub22266oqcxt.onion/10.1128/JVI.63.11.4814-4823.1989 2795719!251119!2795719     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol 1989 ; 63 (11): 4814-23 Nephropedia Template TP {{tp|p=2795719|t=1989. A GC-box motif upstream of the B19 parvovirus unique promoter is important for in vitro transcription |pdf=|usr=}}{{2795719}} gab.com Text A GC-box motif upstream of the B19 parvovirus unique promoter is important for in vitro transcription JO: J Virol http://www.kidney.de/pget.php?&tw=1&pmid=2795719 #FOAvip Nucleotides upstream of the B19 parvovirus P6 promoter affect in vitro transcription in HeLa cell nuclear extracts. Comparison of the relative transcriptional strengths of equimolar mixes of plasmids containing the intact upstream sequence and plasmids containing deletions within these nucleotides identified several regions that affect transcription in vitro. A fragment containing two of five GC-box motifs which correspond to high-affinity SP1-binding sites was shown, by using a gel shift assay, to bind a HeLa cell factor (or factors). DNase I, methylation interference, and methylation protection footprinting demonstrated that the HeLa cell factor(s) bound to one of the two GC-box motifs within this fragment. Mutation of this GC box abolished factor binding and significantly reduces in vitro transcription from the P6 promoter. These results suggest that the B19 parvovirus promoter includes a complex regulatory region containing multiple sequences which affect promoter strength and that the GC-box motif is a major controlling sequence for in vitro transcription. Twit Text FOAVip A GC-box motif upstream of the B19 parvovirus unique promoter is important for in vitro transcription ????J Virol http://www.kidney.de/pget.php?&tw=1&pmid=2795719 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=2795719 #FOAvip English Wikipedia <ref>{{Cite pmid|2795719}}</ref> A GC-box motif upstream of the B19 parvovirus unique promoter is important for in vitro transcription #MMPMID2795719 Blundell MC; Astell CR J Virol 1989[Nov]; 63 (11): 4814-23 PMID2795719show ga Nucleotides upstream of the B19 parvovirus P6 promoter affect in vitro transcription in HeLa cell nuclear extracts. Comparison of the relative transcriptional strengths of equimolar mixes of plasmids containing the intact upstream sequence and plasmids containing deletions within these nucleotides identified several regions that affect transcription in vitro. A fragment containing two of five GC-box motifs which correspond to high-affinity SP1-binding sites was shown, by using a gel shift assay, to bind a HeLa cell factor (or factors). DNase I, methylation interference, and methylation protection footprinting demonstrated that the HeLa cell factor(s) bound to one of the two GC-box motifs within this fragment. Mutation of this GC box abolished factor binding and significantly reduces in vitro transcription from the P6 promoter. These results suggest that the B19 parvovirus promoter includes a complex regulatory region containing multiple sequences which affect promoter strength and that the GC-box motif is a major controlling sequence for in vitro transcription. |*Base Composition[MESH] |*Genes, Viral[MESH] |*Promoter Regions, Genetic[MESH] |*Transcription, Genetic[MESH] |Base Sequence[MESH] |Cell Nucleus/metabolism[MESH] |Cytosine[MESH] |DNA, Viral/*genetics[MESH] |Deoxyribonuclease I[MESH] |Genes, Regulator[MESH] |Guanine[MESH] |HeLa Cells/metabolism[MESH] |Humans[MESH] |Methylation[MESH] |Molecular Sequence Data[MESH] |Mutation[MESH] |Parvoviridae/*genetics[MESH] |Plasmids[MESH] |Templates, Genetic[MESH]A GC-box motif upstream of the B19 parvovirus unique promoter is impor(epmc).pdf DeepDyve Pubget Overpricing