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10.3389/fmicb.2016.01888

http://scihub22266oqcxt.onion/10.3389/fmicb.2016.01888
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27933050!5122736!27933050
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suck abstract from ncbi


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pmid27933050      Front+Microbiol 2016 ; 7 (ä): 1888
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  • An Essential Factor for High Mg(2+) Tolerance of Staphylococcus aureus #MMPMID27933050
  • Armitano J; Redder P; Guimaraes VA; Linder P
  • Front Microbiol 2016[]; 7 (ä): 1888 PMID27933050show ga
  • Internal bacterial concentration of Mg(2+), the most abundant divalent cation in living cells, is estimated to be in the single millimolar range. However, many bacteria will thrive in media with only micromolars of Mg(2+), by using a range of intensely studied and highly efficient import mechanisms, as well as in media with very high magnesium concentration, presumably mediated by currently unknown export mechanisms. Staphylococcus aureus has a particularly high Mg(2+) tolerance for a pathogen, growing unimpaired in up to 770 mM Mg(2+), and we here identify SA0657, a key factor in this tolerance. The predicted domain structure of SA0657 is shared with a large number of proteins in bacteria, archaea and even eukarya, for example CorB from Salmonella and the human CNNM protein family. One of the shared domains, a CBS pair potentially involved in Mg(2+) sensing, contains the conserved Glycine326 which we establish to be a key residue for SA0657 function. In light of our findings, we propose the name MpfA, Magnesium Protection Factor A, for SA0657.
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