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10.1002/jcp.25709 http://scihub22266oqcxt.onion/10.1002/jcp.25709 27925186!ä!27925186 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 302.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 302.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 302.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 302.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 302.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Cell+Physiol 2017 ; 232 (10): 2841-2850 Nephropedia Template TP {{tp|p=27925186|t=2017. Up-Regulation of Transient Receptor Potential Melastatin 6 Channel Expression by Tumor Necrosis Factor-alpha in the Presence of Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor |pdf=|usr=}}{{27925186}} gab.com Text Up-Regulation of Transient Receptor Potential Melastatin 6 Channel Expression by Tumor Necrosis Factor-alpha in the Presence of Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor JO: J Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=27925186 #FOAvip Anti-epidermal growth factor receptor (EGFR) drugs such as erlotinib and gefitinib cause a side effect of hypomagnesemia, but chemotherapy to treat this has not yet been developed. The transient receptor potential melastatin 6 (TRPM6) channel is involved in the reabsorption of Mg(2+) in the renal tubule. We reported previously that the expression of TRPM6 is up-regulated by epidermal growth factor (EGF) in renal tubular epithelial NRK-52E and HEK293 cells. EGF-induced elevation of TRPM6 expression was inhibited by erlotinib, gefitinib, and lapatinib. We found that tumor necrosis factor-alpha (TNF-alpha) increases TRPM6 expression in the presence of erlotinib. Therefore, we investigated what molecules are involved in the up-regulation of TRPM6 expression by TNF-alpha. EGF increased the levels of phosphorylated extracellular signal-regulated kinase 1 and 2 (p-ERK1/2), which were inhibited by erlotinib. TNF-alpha did not change p-ERK1/2 levels, but increased the phosphorylation and nuclear localization of nuclear factor-kappaB (NF-kappaB), which were blocked by the NF-kappaB inhibitors BAY 11-7082 and pyrrolidinedithiocarbamate ammonium. Similarly, luciferase reporter activity of human TRPM6 was increased by TNF-alpha, which was blocked by NF-kappaB inhibitors, and was inhibited by a mutation in the kappaB-binding site in the proximal region of the TRPM6 promoter. A chromatin immunoprecipitation assay revealed that NF-kappaB binds to the kappaB-binding site, which was blocked by NF-kappaB inhibitors. In the presence of erlotinib, TNF-alpha increased Mg(2+) influx, which was blocked by NF-kappaB inhibitors. These results suggest that TNF-alpha reverses the reduction in Mg(2+) reabsorption caused by anti-EGFR drugs. J. Cell. Physiol. 232: 2841-2850, 2017. (c) 2016 Wiley Periodicals, Inc. Twit Text FOAVip Up-Regulation of Transient Receptor Potential Melastatin 6 Channel Expression by Tumor Necrosis Factor-alpha in the Presence of Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor ????J Cell Physiol http://www.kidney.de/pget.php?&tw=1&pmid=27925186 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27925186 #FOAvip English Wikipedia <ref>{{Cite pmid|27925186}}</ref> Up-Regulation of Transient Receptor Potential Melastatin 6 Channel Expression by Tumor Necrosis Factor-alpha in the Presence of Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor #MMPMID27925186 Furukawa C; Fujii N; Manabe A; Matsunaga T; Endo S; Hasegawa H; Ito Y; Yamaguchi M; Yamazaki Y; Ikari A J Cell Physiol 2017[Oct]; 232 (10): 2841-2850 PMID27925186show ga Anti-epidermal growth factor receptor (EGFR) drugs such as erlotinib and gefitinib cause a side effect of hypomagnesemia, but chemotherapy to treat this has not yet been developed. The transient receptor potential melastatin 6 (TRPM6) channel is involved in the reabsorption of Mg(2+) in the renal tubule. We reported previously that the expression of TRPM6 is up-regulated by epidermal growth factor (EGF) in renal tubular epithelial NRK-52E and HEK293 cells. EGF-induced elevation of TRPM6 expression was inhibited by erlotinib, gefitinib, and lapatinib. We found that tumor necrosis factor-alpha (TNF-alpha) increases TRPM6 expression in the presence of erlotinib. Therefore, we investigated what molecules are involved in the up-regulation of TRPM6 expression by TNF-alpha. EGF increased the levels of phosphorylated extracellular signal-regulated kinase 1 and 2 (p-ERK1/2), which were inhibited by erlotinib. TNF-alpha did not change p-ERK1/2 levels, but increased the phosphorylation and nuclear localization of nuclear factor-kappaB (NF-kappaB), which were blocked by the NF-kappaB inhibitors BAY 11-7082 and pyrrolidinedithiocarbamate ammonium. Similarly, luciferase reporter activity of human TRPM6 was increased by TNF-alpha, which was blocked by NF-kappaB inhibitors, and was inhibited by a mutation in the kappaB-binding site in the proximal region of the TRPM6 promoter. A chromatin immunoprecipitation assay revealed that NF-kappaB binds to the kappaB-binding site, which was blocked by NF-kappaB inhibitors. In the presence of erlotinib, TNF-alpha increased Mg(2+) influx, which was blocked by NF-kappaB inhibitors. These results suggest that TNF-alpha reverses the reduction in Mg(2+) reabsorption caused by anti-EGFR drugs. J. Cell. Physiol. 232: 2841-2850, 2017. (c) 2016 Wiley Periodicals, Inc. |Animals[MESH] |Binding Sites[MESH] |Epidermal Growth Factor/pharmacology[MESH] |ErbB Receptors/*antagonists & inhibitors/metabolism[MESH] |Erlotinib Hydrochloride/*toxicity[MESH] |Extracellular Signal-Regulated MAP Kinases/metabolism[MESH] |Gefitinib[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Kidney Tubules/*drug effects/metabolism/physiopathology[MESH] |Lapatinib[MESH] |Magnesium/*metabolism[MESH] |NF-kappa B/metabolism[MESH] |Phosphorylation[MESH] |Promoter Regions, Genetic[MESH] |Protein Binding[MESH] |Protein Kinase Inhibitors/*toxicity[MESH] |Quinazolines/toxicity[MESH] |Rats[MESH] |Renal Reabsorption/*drug effects[MESH] |TRPM Cation Channels/*drug effects/metabolism[MESH] |Time Factors[MESH] |Transfection[MESH] |Tumor Necrosis Factor-alpha/*pharmacology[MESH]Up-Regulation of Transient Receptor Potential Melastatin 6 Channel Exp(epmc).pdf DeepDyve Pubget Overpricing