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10.1111/tbed.12591 http://scihub22266oqcxt.onion/10.1111/tbed.12591 27878975!7169755!27878975     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Transbound+Emerg+Dis 2017 ; 64 (6): 1929-1934 Nephropedia Template TP {{tp|p=27878975|t=2017. Multiplex RT-PCR and Automated Microarray for Detection of Eight Bovine Viruses |pdf=|usr=}}{{27878975}} gab.com Text Multiplex RT-PCR and Automated Microarray for Detection of Eight Bovine Viruses JO: Transbound Emerg Dis http://www.kidney.de/pget.php?&tw=1&pmid=27878975 #FOAvip Microarrays can be a useful tool for pathogen detection as it allow for simultaneous interrogation of the presence of a large number of genetic sequences in a sample. However, conventional microarrays require extensive manual handling and multiple pieces of equipment for printing probes, hybridization, washing and signal detection. In this study, a reverse transcription (RT)-PCR with an accompanying novel automated microarray for simultaneous detection of eight viruses that affect cattle [vesicular stomatitis virus (VSV), bovine viral diarrhoea virus type 1 and type 2, bovine herpesvirus 1, bluetongue virus, malignant catarrhal fever virus, rinderpest virus (RPV) and parapox viruses] is described. The assay accurately identified a panel of 37 strains of the target viruses and identified a mixed infection. No non-specific reactions were observed with a panel of 23 non-target viruses associated with livestock. Vesicular stomatitis virus was detected as early as 2 days post-inoculation in oral swabs from experimentally infected animals. The limit of detection of the microarray assay was as low as 1 TCID(50) /ml for RPV. The novel microarray platform automates the entire post-PCR steps of the assay and integrates electrophoretic-driven capture probe printing in a single user-friendly instrument that allows array layout and assay configuration to be user-customized on-site. Twit Text FOAVip Multiplex RT-PCR and Automated Microarray for Detection of Eight Bovine Viruses ????Transbound Emerg Dis http://www.kidney.de/pget.php?&tw=1&pmid=27878975 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27878975 #FOAvip English Wikipedia <ref>{{Cite pmid|27878975}}</ref> Multiplex RT-PCR and Automated Microarray for Detection of Eight Bovine Viruses #MMPMID27878975 Lung O; Furukawa-Stoffer T; Burton Hughes K; Pasick J; King DP; Hodko D Transbound Emerg Dis 2017[Dec]; 64 (6): 1929-1934 PMID27878975show ga Microarrays can be a useful tool for pathogen detection as it allow for simultaneous interrogation of the presence of a large number of genetic sequences in a sample. However, conventional microarrays require extensive manual handling and multiple pieces of equipment for printing probes, hybridization, washing and signal detection. In this study, a reverse transcription (RT)-PCR with an accompanying novel automated microarray for simultaneous detection of eight viruses that affect cattle [vesicular stomatitis virus (VSV), bovine viral diarrhoea virus type 1 and type 2, bovine herpesvirus 1, bluetongue virus, malignant catarrhal fever virus, rinderpest virus (RPV) and parapox viruses] is described. The assay accurately identified a panel of 37 strains of the target viruses and identified a mixed infection. No non-specific reactions were observed with a panel of 23 non-target viruses associated with livestock. Vesicular stomatitis virus was detected as early as 2 days post-inoculation in oral swabs from experimentally infected animals. The limit of detection of the microarray assay was as low as 1 TCID(50) /ml for RPV. The novel microarray platform automates the entire post-PCR steps of the assay and integrates electrophoretic-driven capture probe printing in a single user-friendly instrument that allows array layout and assay configuration to be user-customized on-site. |Animals[MESH] |Cattle[MESH] |Cattle Diseases/*diagnosis/virology[MESH] |Coinfection/diagnosis/*veterinary/virology[MESH] |Multiplex Polymerase Chain Reaction/methods/*veterinary[MESH] |Oligonucleotide Array Sequence Analysis/methods/*veterinary[MESH] |Reverse Transcriptase Polymerase Chain Reaction/methods/*veterinary[MESH] |Sensitivity and Specificity[MESH] |Virus Diseases/diagnosis/*veterinary/virology[MESH]Multiplex RT-PCR and Automated Microarray for Detection of Eight Bovin(epmc).pdf DeepDyve Pubget Overpricing