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http://scihub22266oqcxt.onion/10.5455/aim.2016.24.248-252
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  • Typing of Methicillin Resistant Staphylococcus Aureus Using DNA Fingerprints by Pulsed-field Gel Electrophoresis #MMPMID27708486
  • Rebic V; Budimir A; Aljicevic M; Bektas S; Vranic SM; Rebic D
  • Acta Inform Med 2016[Jul]; 24 (4): 248-252 PMID27708486garesp_yesshow ga
  • BACKGROUND: Methicillin resistant Staphylococcus aureus (MRSA) is responsible for a wide spectrum of nosocomial and community associated infections worldwide. The aim of this study was to analyze MRSA strains from the general population in Canton Sarajevo, B&H. METHODS: Our investigation including either phenotypic and genotypic markers such as antimicrobial resistance, pulsed-field gel electrophoresis (PFGE), SCC typing, and Panton-Valentine leukocidin (PVL) detection. RESULTS: Antimicrobial susceptibility: all MRSA isolates were resistant to the beta-lactam antibiotics tested, and all isolates were susceptible trimethoprim sulphamethoxazole, rifampicin, fusidic acid, linezolid and vancomycin. Sixty-eight per cent of the MRSA isolates were resistant to erythromycin, 5% to clindamycin, 5% to gentamicin and 4% to ciprofloxacin. After the PFGE analysis, the isolates were grouped into five similarity groups: A-E. The largest number of isolates belonged to one of two groups: C: 60 (60%) and D: 27 (27%). In both groups C and D, SCCmec type IV was predominant (60% and 88, 8%, respectively). A total of 24% of the isolates had positive expression of PVL genes, while 76% showed a statistically significantly greater negative expression of PVL genes. CONCLUSION: SCCmec type IV, together with the susceptibility profile and PFGE grouping, is considered to be typical of CA-MRSA.
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    248 4.24 2016