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10.1002/tox.22293 http://scihub22266oqcxt.onion/10.1002/tox.22293 27297870!ä!27297870 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 298.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 298.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 298.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 298.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 298.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 298.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Environ+Toxicol 2017 ; 32 (3): 918-930 Nephropedia Template TP {{tp|p=27297870|t=2017. Andrographolide inhibits hypoxia-induced HIF-1alpha-driven endothelin 1 secretion by activating Nrf2/HO-1 and promoting the expression of prolyl hydroxylases 2/3 in human endothelial cells |pdf=|usr=}}{{27297870}} gab.com Text Andrographolide inhibits hypoxia-induced HIF-1alpha-driven endothelin 1 secretion by activating Nrf2/HO-1 and promoting the expression of prolyl hydroxylases 2/3 in human endothelial cells JO: Environ Toxicol http://www.kidney.de/pget.php?&tw=1&pmid=27297870 #FOAvip Andrographolide, the main bioactive component of the medicinal plant Andrographis paniculata, has been shown to possess potent anti-inflammatory activity. Endothelin 1 (ET-1), a potent vasoconstrictor peptide produced by vascular endothelial cells, displays proinflammatory property. Hypoxia-inducible factor 1alpha (HIF-1alpha), the regulatory member of the transcription factor heterodimer HIF-1alpha/beta, is one of the most important molecules that responds to hypoxia. Changes in cellular HIF-1alpha protein level are the result of altered gene transcription and protein stability, with the latter being dependent on prolyl hydroxylases (PHDs). In this study, inhibition of pro-inflammatory ET-1 expression and changes of HIF-1alpha gene transcription and protein stability under hypoxia by andrographolide in EA.hy926 endothelial-like cells were investigated. Hypoxic conditions were created using the hypoxia-mimetic agent CoCl(2.) We found that hypoxia stimulated the production of reactive oxygen species (ROS), the expression of HIF-1alpha mRNA and protein, and the expression and secretion of ET-1. These effects, however, were attenuated by co-exposure to andrographolide, bilirubin, and RuCO. Silencing Nrf2 and heme oxygenase 1 (HO-1) reversed the inhibitory effects of andrographolide on hypxoia-induced HIF-1alpha mRNA and protein expression. Moreover, andrographolide increased the expression of prolyl hydroxylases (PHD) 2/3, which hydroxylate HIF-1alpha and promotes HIF-1alpha proteasome degradation, with an increase in HIF-1alpha hydroxylation was noted under hypoxia. Inhibition of p38 MAPK abrogated the hypoxia-induced increases in HIF-1alpha mRNA and protein expression as well as ET-1 mRNA expression and secretion. Taken together, these results suggest that andrographolide suppresses hypoxia-induced pro-inflammatory ET-1 expression by activating Nrf2/HO-1, inhibiting p38 MAPK signaling, and promoting PHD2/3 expression. (c) 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 918-930, 2017. Twit Text FOAVip Andrographolide inhibits hypoxia-induced HIF-1alpha-driven endothelin 1 secretion by activating Nrf2/HO-1 and promoting the expression of prolyl hydroxylases 2/3 in human endothelial cells ????Environ Toxicol http://www.kidney.de/pget.php?&tw=1&pmid=27297870 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27297870 #FOAvip English Wikipedia <ref>{{Cite pmid|27297870}}</ref> Andrographolide inhibits hypoxia-induced HIF-1alpha-driven endothelin 1 secretion by activating Nrf2/HO-1 and promoting the expression of prolyl hydroxylases 2/3 in human endothelial cells #MMPMID27297870 Lin HC; Su SL; Lu CY; Lin AH; Lin WC; Liu CS; Yang YC; Wang HM; Lii CK; Chen HW Environ Toxicol 2017[Mar]; 32 (3): 918-930 PMID27297870show ga Andrographolide, the main bioactive component of the medicinal plant Andrographis paniculata, has been shown to possess potent anti-inflammatory activity. Endothelin 1 (ET-1), a potent vasoconstrictor peptide produced by vascular endothelial cells, displays proinflammatory property. Hypoxia-inducible factor 1alpha (HIF-1alpha), the regulatory member of the transcription factor heterodimer HIF-1alpha/beta, is one of the most important molecules that responds to hypoxia. Changes in cellular HIF-1alpha protein level are the result of altered gene transcription and protein stability, with the latter being dependent on prolyl hydroxylases (PHDs). In this study, inhibition of pro-inflammatory ET-1 expression and changes of HIF-1alpha gene transcription and protein stability under hypoxia by andrographolide in EA.hy926 endothelial-like cells were investigated. Hypoxic conditions were created using the hypoxia-mimetic agent CoCl(2.) We found that hypoxia stimulated the production of reactive oxygen species (ROS), the expression of HIF-1alpha mRNA and protein, and the expression and secretion of ET-1. These effects, however, were attenuated by co-exposure to andrographolide, bilirubin, and RuCO. Silencing Nrf2 and heme oxygenase 1 (HO-1) reversed the inhibitory effects of andrographolide on hypxoia-induced HIF-1alpha mRNA and protein expression. Moreover, andrographolide increased the expression of prolyl hydroxylases (PHD) 2/3, which hydroxylate HIF-1alpha and promotes HIF-1alpha proteasome degradation, with an increase in HIF-1alpha hydroxylation was noted under hypoxia. Inhibition of p38 MAPK abrogated the hypoxia-induced increases in HIF-1alpha mRNA and protein expression as well as ET-1 mRNA expression and secretion. Taken together, these results suggest that andrographolide suppresses hypoxia-induced pro-inflammatory ET-1 expression by activating Nrf2/HO-1, inhibiting p38 MAPK signaling, and promoting PHD2/3 expression. (c) 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 918-930, 2017. |Cell Hypoxia[MESH] |Cell Line[MESH] |Cell Survival/drug effects[MESH] |Cobalt/toxicity[MESH] |Diterpenes/*pharmacology[MESH] |Endothelial Cells/cytology/metabolism[MESH] |Endothelin-1/genetics/*metabolism[MESH] |Heme Oxygenase-1/antagonists & inhibitors/genetics/*metabolism[MESH] |Humans[MESH] |Hydroxylation[MESH] |Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism[MESH] |NF-E2-Related Factor 2/antagonists & inhibitors/genetics/*metabolism[MESH] |Prolyl Hydroxylases/genetics/*metabolism[MESH] |RNA Interference[MESH] |RNA, Messenger/metabolism[MESH] |RNA, Small Interfering/metabolism[MESH] |Reactive Oxygen Species/metabolism[MESH] |Signal Transduction/drug effects[MESH]Andrographolide inhibits hypoxia-induced HIF-1alpha-driven endothelin (epmc).pdf DeepDyve Pubget Overpricing