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10.3892/mmr.2016.5324 http://scihub22266oqcxt.onion/10.3892/mmr.2016.5324 27220406!4918618!27220406     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 284.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Med+Rep 2016 ; 14 (1): 689-96 Nephropedia Template TP {{tp|p=27220406|t=2016. Notch signaling represses hypoxia-inducible factor-1alpha-induced activation of Wnt/beta-catenin signaling in osteoblasts under cobalt-mimicked hypoxia |pdf=|usr=}}{{27220406}} gab.com Text Notch signaling represses hypoxia-inducible factor-1alpha-induced activation of Wnt/beta-catenin signaling in osteoblasts under cobalt-mimicked hypoxia JO: Mol Med Rep http://www.kidney.de/pget.php?&tw=1&pmid=27220406 #FOAvip The modification of Wnt and Notch signaling pathways by hypoxia, and its association with osteoblast proliferation and apoptosis remain to be fully elucidated. To investigate Wnt-Notch crosstalk, and its role in hypoxia-induced osteoblast proliferation and apoptosis regulation, the present study investigated the effects of cobalt?mimicked hypoxia on the mouse pre-osteoblast-like cell line, MC3T3?E1, when the Notch signals were repressed using a gamma?secretase inhibitor DAPT. The data showed that the cobalt?mimicked hypoxia suppressed cell proliferation under normal conditions, but increased cell proliferation under conditions of Notch repression, in a concentration?dependent manner. The results of western blot and reverse transcription?quantitative polymerase chain reaction analyses showed that the cobalt treatment increased the levels of activated beta?catenin protein and the expression levels of the target genes, axis inhibition protein 2 and myelocytomatosis oncogene, under DAPT?induced Notch repression. However, no significant changes were found in the expression levels of the Notch intracellular domain protein or the Notch target gene, hes1. In a beta?catenin gene?knockdown experiment, the proliferation of the MC3T3?E1 cells under hypoxia were decreased by DAPT treatment, and knockdown of the expression of hypoxia?inducible factor?1alpha (HIF?1alpha) suppressed the cobalt?induced increase in Wnt target gene levels. No significant difference in cell proliferation rate was found following DAPT treatment when the expression of HIF?1alpha was knocked down. The results of the present study showed the opposing effects of Wnt and Notch signaling under cobalt?mimicked hypoxia, which were partially regulated by HIF?1alpha, The results also showed that osteoblast proliferation was dependent on Wnt-Notch signal crosstalk. Twit Text FOAVip Notch signaling represses hypoxia-inducible factor-1alpha-induced activation of Wnt/beta-catenin signaling in osteoblasts under cobalt-mimicked hypoxia ????Mol Med Rep http://www.kidney.de/pget.php?&tw=1&pmid=27220406 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27220406 #FOAvip English Wikipedia <ref>{{Cite pmid|27220406}}</ref> Notch signaling represses hypoxia-inducible factor-1alpha-induced activation of Wnt/beta-catenin signaling in osteoblasts under cobalt-mimicked hypoxia #MMPMID27220406 Li CT; Liu JX; Yu B; Liu R; Dong C; Li SJ Mol Med Rep 2016[Jul]; 14 (1): 689-96 PMID27220406show ga The modification of Wnt and Notch signaling pathways by hypoxia, and its association with osteoblast proliferation and apoptosis remain to be fully elucidated. To investigate Wnt-Notch crosstalk, and its role in hypoxia-induced osteoblast proliferation and apoptosis regulation, the present study investigated the effects of cobalt?mimicked hypoxia on the mouse pre-osteoblast-like cell line, MC3T3?E1, when the Notch signals were repressed using a gamma?secretase inhibitor DAPT. The data showed that the cobalt?mimicked hypoxia suppressed cell proliferation under normal conditions, but increased cell proliferation under conditions of Notch repression, in a concentration?dependent manner. The results of western blot and reverse transcription?quantitative polymerase chain reaction analyses showed that the cobalt treatment increased the levels of activated beta?catenin protein and the expression levels of the target genes, axis inhibition protein 2 and myelocytomatosis oncogene, under DAPT?induced Notch repression. However, no significant changes were found in the expression levels of the Notch intracellular domain protein or the Notch target gene, hes1. In a beta?catenin gene?knockdown experiment, the proliferation of the MC3T3?E1 cells under hypoxia were decreased by DAPT treatment, and knockdown of the expression of hypoxia?inducible factor?1alpha (HIF?1alpha) suppressed the cobalt?induced increase in Wnt target gene levels. No significant difference in cell proliferation rate was found following DAPT treatment when the expression of HIF?1alpha was knocked down. The results of the present study showed the opposing effects of Wnt and Notch signaling under cobalt?mimicked hypoxia, which were partially regulated by HIF?1alpha, The results also showed that osteoblast proliferation was dependent on Wnt-Notch signal crosstalk. |*Signal Transduction[MESH] |*Wnt Signaling Pathway[MESH] |Amyloid Precursor Protein Secretases/metabolism[MESH] |Animals[MESH] |Cell Line[MESH] |Cell Proliferation/drug effects[MESH] |Cobalt/*pharmacology[MESH] |Gene Expression Regulation/drug effects[MESH] |Humans[MESH] |Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism[MESH] |Hypoxia/metabolism[MESH] |Mice[MESH] |Osteoblasts/*drug effects/*metabolism[MESH] |Receptors, Notch/*metabolism[MESH] |Wnt Proteins/metabolism[MESH]Notch signaling represses hypoxia-inducible factor-1alpha-induced acti(epmc).pdf DeepDyve Pubget Overpricing